Total Degradation of Pentachloroethane by an Engineered Alcaligenes Strain Expressing a Modified Camphor Monooxygenase and a Hybrid Dioxygenase
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概要
- 論文の詳細を見る
We engineered biphenyl-degrading Alcaligenes sp. strain KF711 for total degradation of pentachloroethane (PCA), which expresses a modified camphor monooxygenase and a hybrid dioxygenase consisting of TodC1 (a large subunit of toluene dioxygenase of Pseudomonas putida F1) and BphA2-BphA3-pbhA4 (a small subunit, ferredoxin and ferredoxin reductase of biphenyl dioxygenase, respectively, in strain KF707). Modified camphor monooxygenase genes (camCAB) were supplied as a plasmid and the todC1 gene was integrated within the chromosomal bph gene cluster by a single crossover recombination. The resultant strain KF711S-3cam dechlorinated PCA to trichloroethene by the action of the modified camphor monooxygenase under anaerobic conditions. The same strain subsequently degraded trichloroethene formed oxidatively by the action of the Tol-Bph hybrid dioxygenase under aerobic conditions. Thus sequential anaerobic and aerobic treatments of the KF711S-3cam resting cells resulted in efficient and total degradation of PCA.
- 社団法人 日本農芸化学会の論文
- 2004-06-23
著者
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GOTO Masao
National Agriculture and Food Research Organization National Food Research Institute
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Goto Masatoshi
Dep. Of Bioscience And Biotechnology Kyushu Univ.
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Goto Masatoshi
Department Of Agricultural Chemistry Kyushu University
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Furukawa Kensuke
Department Of Bioscience And Biotechnology Faculty Of Agriculture Kyushu University
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Furukawa Kensuke
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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Furukawa Kensuke
Department Of Food And Bioscience Faculty Of Food Science And Nutrition Beppu University
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IWAKIRI Ryo
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
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Iwakiri Ryo
Department Of Bioscience And Biotechnology Faculty Of Agriculture Kyushu University
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Furukawa Kensuke
Lab. Appl. Microbiol. Grad. Sch. Biores. Bioenviron. Sci. Kyushu Univ.
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YOSHIHIRA Kunichika
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
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FUTAGAMI Taiki
Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
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Yoshihira Kunichika
Department Of Bioscience And Biotechnology Faculty Of Agriculture Kyushu University
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Futagami Taiki
Department Of Bioscience And Biotechnology Faculty Of Agriculture Kyushu University
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