44(P-7) ビフェニル分解系遺伝子群を導入した大腸菌による芳香族化合物のhigh throughput bioconversion : "BioCombiChem"の展開(ポスター発表の部)

概要

論文の詳細を見る
We intend to develop a system for the comprehensive bioconversion of a series of organic chemicals through the living cells of recombinant microbes. This intention attempts to perform combinatorial chemistry by using biological functions to link the diversity of genes (DNA) to that of chemicals. The biphenyl-degrading (metabolizing) pathway progresses through four enzymes, biphenyl dioxygenase (BphA), dihydrodiol dehydrogenase (BphB), 2,3-dihydrodiol dehydrogenase (BphC), and hydrolase (BphD). In the 2000's annual meeting, we reported efficient biotransformation of various aromatic compounds to produce the corresponding cis-dihydrodiols by using Escherichia coli carrying the evolved BphA [BphA (2072); BphA1(2072)BphA2A3A4] which has a wide substrate specificity. Recently, we succeeded in a series of comprehensive biotransformation of various aromatics by using E. coli carrying the genes cluster encoding biphenyl-degrading enzymes systematically as follows: 1) BphA (2072) + BphB: Aromatic compounds were converted to the corresponding vicinal diol (catechol) derivatives. 2) BphA (2072) + BphB + BphC: Aromatic compounds were converted to the corresponding picolinic acid derivatives. 3) BphA (2072) + BphB + BphC + BphD: Aromatic compounds were converted to the corresponding carboxylic acid derivatives. The above biological technique seems to be simpler and effective methods, and is likely to complement the tequniques of chemical synthesis. Additionally, the products converted by BphA and BphB showed potent antioxidative activities, which means the probability for the creation of bioactive compounds using this method. Such biological technologies that we call "BioCombiChem" (Biology-based Combinatorial Chemistry) make it practical to synthesize chemicals which are very difficult or impossible to synthesize by chemical methods.
2004-10-01

44(P-7) ビフェニル分解系遺伝子群を導入した大腸菌による芳香族化合物のhigh throughput bioconversion : "BioCombiChem"の展開(ポスター発表の部)

スポンサーリンク

概要

著者

関連論文

スポンサーリンク