Oxygenation Reactions of Various Tricyclic Fused Aromatic Compounds Using Escherichia coli and Streptomyces lividans Transformants Carrying Several Arene Dioxygenase Genes(Microbiology & Fermentation Technology)
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概要
- 論文の詳細を見る
Bioconversion (biotransformation) experiments on arenes (aromatic compounds), including various tricyclic fused aromatic compounds such as fluorene, dibenzofuran, dibenzothiophene, carbazole, acridene, and phenanthridine, were done using the cells of Escherichia coli transformants expressing several arene dioxygenase genes. E. coli carrying the phenanthrene dioxygenase (phdABCD) genes derived from the marine bacterium Nocardioides sp. strain KP7 converted all of these tricyclic aromatic compounds, while E. coli carrying the Pseudomonas putida Fl toluene dioxygenase (todC1C2BA) genes or the P. pseudoalcaligenes KF707 biphenyl dioxygenase (bphA1A2A3A4) genes was not able to convert these substrates. Surprisingly, E. coli carrying hybrid dioxygenase (todCl : : bphA2A3A4) genes with a subunit substitution between the toluene and biphenyl dioxygenases was able to convert fluorene, dibenzofuran, and dibenzothiophene. The cells of a Streptomyces lividans transformant carrying the phenanthrene dioxygenase genes were also evaluated for bioconversion of various tricyclic fused aromatic compounds. The ability of this actinomycete in their conversion was similar to that of E. coli carrying the corresponding genes. Products converted from the aromatic compounds with these recombinant bacterial cells were purified by column chromatography on silica gel, and identified by their MS and ^1H and ^<13>C NMR analyses. Several products, e. g., 4-hydroxyfluorene converted from fluorene, and cis-1,2-dihydroxy-1,2-dihydrophenanthridine, cis-9,10-dihydroxy-9,10-dihydrophenanthridine, and 10-hydroxyphenaiithridine, which were converted from phenanthridine, were novel compounds.
- 社団法人日本農芸化学会の論文
- 2001-11-23
著者
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MISAWA Norihiko
Central Laboratories for Frontier Technology, Kirin Holdings Co., Ltd.
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林 正弘
東京薬大・薬
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Furihata K
Univ. Tokyo Tokyo Jpn
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Hayashi Miki
Central Laboratories For Key Technology Kirin Brewery Co. Ltd.
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HORINOUCHI Sueharu
Department of Biotechnology, University of Tokyo
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SHINDO Kazutoshi
Pharmaceutical Research Laboratory, Kirin Brewery Co. Ltd.
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Iguchi Kazuo
Tokyo University Of Pharmacy And Life Science
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Iguchi Kazuo
Tokyo College Of Pharmacy
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Furihata Kazuo
Division Of Agriculture And Agricultural Life Sciences
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Hayashi M
Institute Of Environmental Science For Human Life Ochanomizu University
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OHNISHI Yasuo
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tok
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CHUN Hyo-Kon
Department of Biotechnology, Graduate School of Agriculture and Life Sciences, The University of Tok
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TAKAHASHI Haruko
Tokyo University of Pharmacy and Life Science
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SAITO Atsushi
Marine Biotechnology Institute
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FURUKAWA Kensuke
Division of Bioresource and Bioenvironmental Sciences, Kyushu University
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HARAYAMA Shigeaki
Marine Biotechnology Institute
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CHUN Hyo-Kon
Korea Research Institute of Bioscience and Biotechnology
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Misawa Norihiko
Marine Biotechnology Institute:(present Office)central Laboratories For Frontier Technology Kirin Ho
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Misawa Norihiko
Central Laboratories For Frontier Technology Kirin Holdings Co. Ltd.
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Furukawa Kensuke
Division Of Bioresource And Bioenvironmental Sciences Kyushu University
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Furukawa Kensuke
Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu University
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Shindo Kazutoshi
Pharmaceutical Research Laboratories Kirin Brewery Co. Ltd.
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Horinouchi Sueharu
Department Of Biotechnology Graduate School Of Agricultural And Life Sciences The University Of Toky
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Horinouchi Sueharu
Department Of Agricultural Chemistry Faculty Of Agriculture University Of Tokyo
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Horinouchi Sueharu
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Horinouchi Sueharu
Dep. Of Biotechnology Graduate School Of Agriculture And Life Sciences Univ. Of Tokyo
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Hayashi Mizuki
Institute Of Environmental Science For Human Life Ochanomizu University
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Furukawa K
Graduate School Of Bioresource And Bioenvironmental Sciences Kyushu University
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Ohnishi Y
Department Of Biotechnology The University Of Tokyo
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Murata H
Univ. Miyazaki Miyazaki Jpn
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Misawa N
Marine Biotechnology Institute:(present Office)central Laboratories For Frontier Technology Kirin Ho
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Horinouchi S
Department Of Biotechnology The University Of Tokyo
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Harayama S
Marine Biotechnol. Inst. Iwate Jpn
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Shindo K
Department Of Food And Nutrition Japan Women's University
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Ohnishi Yasuo
Department Of Biotechnolog Graduate School Of Agriculture And Life Sciences The University Of Tokyo
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Shindo Kazutoshi
Japan Women's University
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Misawa Norihiko
Central Laboratories For Frontier Technol. Kirin Holdings Co. Ltd.
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Saito A
Marine Biotechnology Institute
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