Biphenyl-inducible Promoters in a Polychlorinated Biphenyl-degrading Bacterium, Rhodococcus sp. RHA1

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概要

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• Five transcriptional promoters of biphenyl-degradation genes in Rhodococcus sp. RHA1 were characterized. We newly identified the etbA4 promoter region, which was located adjacent upstream from a ferredoxin reductase gene, etbA4 and a dihydrodiol dehydrogenase gene, bphB2. The etbA4 promoter activity was determined in RHA1 using a promoter probe vector with a luxAB luciferase reporter gene, and was induced by a variety of aromatic compounds as well as the bphA1, ebdA1, etbA1, and etbD1 promoters. All these promoters were induced by aromatic compounds in a closely related heterologous host, R. erythropolis IAM1399 in the presence of RHA1 bphST genes, suggesting that these five promoters are under the control of bphST-coding two-component regulatory system. Sequence comparison of the bphA1 promoter with the ebdA1 and etbA1 promoters, whose transcription starts were determined by primer extension analysis, revealed a consensus sequence centering 42-bp upstream from the transcription start. This consensus was also conserved in the etbA4 and etbD1 promoters, and deletions of the bphA1 promoter affecting the consensus impaired inducible promoter activity. These results suggest that this consensus plays a role in transcription induction and/or the promotion of biphenyl degradation genes in RHA1.

• 社団法人 日本農芸化学会の論文
• 2004-06-23

著者

• Fukuda Masao

  Nagaoka University of Technology

• Masai Eiji

  Nagaoka University of Technology

• MASAI Eiji

  Department of Bioengineering, Nagaoka University of Technology

• YAMADA Akihiro

  Department of Functional Materials Science, Saitama University

• Masai E

  Department Of Bioengineering Nagaoka University Of Technology

• Masai Eiji

  Dep. Of Bioengineering Nagaoka Univ. Of Technol.

• Masai Eiji

  Department Of Bioengineering Nagaoka University Of Engineering

• Fukuda Masao

  Department Of Bioengineering Nagaoka University Of Technology

• Fukuda Masao

  Dep. Of Bioengineering Nagaoka Univ. Of Technol.

• TAKEDA Hisashi

  Department of Biophysical Genetics, Kanazawa University Graduate School of Medicine

• Fukuda M

  Nagaoka University Of Technology

• MIYAUCHI Keisuke

  Department of Civil and Environmental Engineering, Tohoku Gakuin University

• Sakai Miho

  Dept. Appl. Microbial Technology Kumamoto Institute Of Technology

• Fukuda Masao

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• HARA Naho

  Department of Bioengineering, Nagaoka University of Technology

• SAKAI Masayuki

  Department of Bioengineering, Nagaoka University of Technology

• Takeda Hisashi

  Department Of Biophysical Genetics Kanazawa University Graduate School Of Medicine

• Miyauchi Keisuke

  Department Of Bioengineering Nagaoka University Of Technology

• Hara Naho

  Department Of Bioengineering Nagaoka University Of Technology

• Yamada A

  Department Of Bioengineering Nagaoka University Of Technology

• Miyauchi Keisuke

  Department Of Civil And Environmental Engineering Tohoku Gakuin University

• Tanaka Hiroshi

  National Institute Of Agrobiological Sciences

• Yamada Akihiro

  Department Of Chemistry Graduate School Of Science

• YAMADA AKIHIRO

  Department of Bioengineering, Nagaoka University of Technology

• Takeda Hisashi

  National Institute of Agrobiological Sciences

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