Three-dimensional Structure of 2,3-Dihydroxybiphenyl Dioxygenase (BphC enzyme) from Pseudomonas sp. Strain KKS102 Having Polychlorinated Biphenyl (PCB)-Degrading Activity.

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概要

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• The three-dimensional structure of an enzyme, 2, 3-dihydroxybiphenyl dioxygenase, from Pseudomonas sp. strain KKS102 has been solved by X-ray crystal structure analysis. The enzyme conventionally called "BphC" is an important member in the biodegradation pathway for PCBs (polychlorinated biphenyls) which are the widely distributed environmental pollutants. The BphC enzyme is an oligomeric enzyme made up of eight identical subunits of 292 amino acid residues. Each subunit consists of two domains: Domain 1(residues 1 to 135) and Domain 2 (resiuues 136 to 292). Each domain consists of two repetitions of a unique folding motif each consisting of ca. 55 amino acid residues. The unique motif may be classified into an α-β sandwich structure having a "βαβββ" type topology. In the active site of each subunit, one Fe ion surrounded by five ligands roughly arranged in a trigonal bipyramidal geometry was found.

• 日本学士院の論文

著者

• Fukuda Masao

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• Yano Keiji

  Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo

• MITSUI Yukio

  Department of BioEngineering Nagaoka University of Technology

• Narita Hiroki

  Department Of Industrial Chemistry Seikei University

• SENDA Toshiya

  Department of BioEngineering, Nagaoka University of Technology

• Yamamoto Takeshi

  Department Of Agricultural Biochemistry And Biotechnology Tottori University

• KIMBARA Kazuhide

  Department of BioEngineering, Nagaoka University of Technology

• SUGIYAMA Kazuyuki

  Department of BioEngineering, Nagaoka University of Technology

• NARITA Hiroki

  Department of BioEngineering, Nagaoka University of Technology

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