Photosynthetic Conversion of Carbon Dioxide to Ethylene by the Recombinant Cyanobacterium, Synechococcus sp. PCC 7942,Which Harbors a Gene for the Ethylene-Forming Enzyme of Pseudomonas syringae
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概要
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Photoynthetic conversion of carbon dioxide to ehtylene was studied using the recombinant cyanobacterium, Synechococcus sp. strain PCC 7942 R2-SPc which expresses the ethylene-forming enzyme (EFE) from Pseudomonas syringae pv. phaseolicola PK2. The gene encoding the EFE (efe gene) from P. syringae was introduced into the cyanobacterium utilizing the pUC303 shuttle vector into which the efe gene was placed under the control of various transcriptional signals, i.e., the native promoter and terminator of the efe gene (pUC303-EFE02), the Escherichia coli lacZ promoter and the efe gene terminator (pUC303-EFE10 and pUC303-EFE30), or the promoter and terminator of the psbAI gene from Synechococcus sp. PCC7942 which codes for the D1 protein in photosystem II(pEXE3). Among these configurations, EFE activity measured in the cell-free extracts of transformants that harbored the pEXE3 was highest. However, ethylene production in vivo of the transformants carying pEXE3 declined with the number of generations, because homologous recombination of DNA sequences on the pEXE3 plasmid and host chromosomal psbAI locus took place.Deletion of the 5'-upstream region of the psbAI promoter and the 3'-downstream region of the psbAI terminator in pEXE3 resulted in pEXE3Δ8 which showed the higest level of ethylene-forming activity, although the latter plasmid was still unstable with a half-life of only 12 generations. The amount of carbon incorporated into ethylene was calculated as a percentage of the total carbon fixed, the maximum value of which was 5.84% in the recombinant cyanobacterium harboring pUC303-EFE03.
- 社団法人日本生物工学会の論文
- 1997-11-25
著者
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MATSUOKA MASAYOSHI
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo Universi
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OGAWA TAKAHIRA
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo Universi
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Ogawa T
Shinshu Univ. Nagano Jpn
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Fukuda H
Kobe Univ. Kobe
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Fukuda Hideki
Department Of Molecular Science And Material Engineering Graduate School Of Science And Technology K
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Ogawa T
Kyoto Univ. Kyoto Jpn
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Ogawa Takahiro
Department Of Biological Science And Technology Science University Of Tokyo
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Ogawa T
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Matsuoka M
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Matsuoka Masayoshi
Dept. Applied Microb. Technol. Sojo Univ.
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FUKUDA HIDEO
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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SAKAI MIHO
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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Fukuda H
Kobe Univ. Kobe Jpn
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Sakai Miho
Dept. Appl. Microbial Technology Kumamoto Institute Of Technology
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Ogawa T
Kumamoto Inst. Technol. Kumamoto Jpn
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Ogawa Takahira
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Ogawa Takahira
Dept. Appl. Microbial Technology Kumamoto Institute Of Technology
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Matsuoka Masayoshi
Dept. Appl. Microbial Technology Kumamoto Institute Of Technology
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Matsuoka Masayoshi
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Fukuda Hideo
Department Of Applied Microbial Technology The Kumamoto Institute Of Technolgy
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Fukuda Hideo
Dept. Appl. Microbial Technology Kumamoto Institute Of Technology
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Ogawa Tadashi
Graduate School Of Bioagricultural Sciences Nagoya University
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Fukuda Hideo
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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