Purification and Characterization of Soybean Allergen Gly m Bd 28K

スポンサーリンク

概要

• 論文の詳細を見る

• At least 15 allergenic proteins have been found in soybean using the sera of soybean-sensitive patients with atopic dermatitis [T. Ogawa et al., J. Nutr. Sci. Vitaminol., 35, 555-565 (1991)]. In the present study, a monoclonal antibody (mAb) against Gly m Bd 28K, one of the major allergens of soybean, was prepared, and Gly m Bd 28K was purified from defatted soybean tlakes by five purification steps, including immunoaffinity chromatography with the mAb as a ligand. The purified allergen was found to be a glycoprotein with a molecular mass of 26 kDa. During the purification process the allergen was converted to more acidic proteins with the same molecular mass, suggesting that the allergen is unstable. The sugar composition and amino acid sequence of Gly m Bd 28K suggest that the allergen is a new glycoprotein with an Asn-linked sugar moiety. The distribution of the allergen in soybean products was examined by an immunoblotting technique with the mAb.

• 社団法人日本農芸化学会の論文
• 1997-06-23

著者

• NISHIKAWA Kiyoshi

  Department of Pediatrics, National Kagawa Children's Hospital

• 西川 喜良

  甲南大学理学部

• HIEMORI Miki

  Department of Nutritional Science, Okayama Prefectural University

• TSUJI Hideaki

  Department of Nutritional Science, Okayama Prefectural University

• Kimoto Masumi

  Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University

• Ogawa T

  Shinshu Univ. Nagano Jpn

• Tsuji Hiroaki

  Nisshin Oil Mills Ltd.

• Ogawa T

  Kyoto Univ. Kyoto Jpn

• Ogawa Takahiro

  Department Of Biological Science And Technology Science University Of Tokyo

• Tsuji H

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Tsuji Hideaki

  Graduate School Of Health And Welfare Science Okayama Prefectural University

• Tsuji Hideaki

  Department Of Chemistry Faculty Of Education Gifu University

• Ogawa T

  Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University

• Kimoto Masumi

  Department Of Nutritional Science Okayama Prefectural University

• BANDO Noriko

  Department of Nutrition, School of Medicine, The University of Tokushima

• OGAWA Tadashi

  Department of Nutrition, School of Medicine, The University of Tokushima

• YAMANISHI Rintaro

  Department of Nutrition, School of Medicine, The University of Tokushima

• Tsuji Hideaki

  Department Of Nutritional Science Okayama Prefectural University

• Ogawa Takahira

  Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University

• Ogawa Takahira

  Dept. Appl. Microbial Technology Kumamoto Institute Of Technology

• Nishikawa Kiyoshi

  Department Of Electrical Engineering Tokyo Metropolitan University

• Nishikawa K

  Department Of Pediatrics National Kagawa Children's Hospital

• Nishikawa K

  Course. Of Info. And Commn. Sys. Eng. Tokyo Metro. Univ.

• Ogawa Tadashi

  Graduate School Of Bioagricultural Sciences Nagoya University

• Ogawa Tadashi

  Department Of Legal Medicine Aichi Medical University School Of Medicine

• Noriko Bando

  Department Of Food Science Graduate School Of Nutrition And Biosciences The University Of Tokushima

• Yamanishi Rintaro

  Department Of Food Science Graduate School Of Nutrition And Biosciences The University Of Tokushima

• Yamanishi Rintaro

  Laboratory Of Nutritonal Chemistry Department Of Food Science And Technology Faculty Of Agriculture

• Horiguchi M

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Bando Noriko

  Department Of Food Science Graduate School Of Nutrition And Bioscience The University Of Tokushima

• Kimoto M

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Nishikawa Kiyoshi

  Department Of Chemistry Faculty Of Science Kanazawa University

• Kimoto Masumi

  Department of Immunology and Host Defenses, Ehime University Graduate School of Medicine

• Ogawa Tadashi

  Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University

• Hiemori Miki

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University

• HIEMORI Miki

  Department of Nutrition, School of Medicine, The University of Tokushima

関連論文

• Questionnaire Survey on Asthma Management of Japanese Allergists II. Treatment methods
• Questionnaire Survey on Asthma Management of Japanese Allergists I. Diagnosis, patient education and management
• 大学基礎物理学実験一題目の改良による成果と今後の問題点
• B316 大学基礎物理学実験一題目の改良とその成果
• Development of a Method for the Determination of γ-Aminobutyric Acid in Foodstuffs
• D304 教育を目的としたデータベースとその利用
• Effect of Current Density and Organic Additives on the Texture and Hardness of Ni Electrodeposited from Sulfamate and Watt's Solutions
• Isolation and molecular cloning of a major wheat allergen, Tri a Bd 27K
• D205 ステートメントとプロトコールの研究(5)
• B109 理解状態およびそのプロセスの科学分析(7)
• E204 ステートメントとプロトコールの研究(4)
• A208 理解状態およびそのプロセスの科学的分析(6)
• F-5 ステートメントとプロトコールの研究(2)
• E-18 理解状態およびそのプロセスの科学的分析(4)
• K-2 理解状態およびそのプロセスの科学的分析(3)
• CHACALTAYA Emulsion Chamber Experiment
• C31 教育効果評価のためのデータ・ベースの活用
• 教育効果評価のためのデータ・ベース III
• B313 教育効果評価のためのデータ・ベース II
• F223 概念チャートデータベース
• F222 教育効果評価のためのデータベース
• D107 教育支援のためのミニ・データベースの開発
• 2Fp-2 ステートメントとプロトコールの研究 (3)
• 1CP-4 理解状態およびそのプロセスの科学的分析 (5)
• J-5 ステートメントとプロトコールの研究(1)
• C-23 理解状態およびそのプロセスの科学的分析
• 科学教育についてのインフォーメイション・アナリシス 第3報
• A-27 理解状態およびそのプロセスの科学的分析(2)
• Purification and Partial Characterization of Acetyl-CoA Synthetase in Rat Liver Mitochondria
• Structural and Immunochemical Homologies between Foxtail Millet Glutelin 60 kDa and Starch Granule-bound Starch Synthase Proteins from Rice, Barley, Corn and Wheat Grains
• Efficient Extraction and Some Properties of Storage Proteins (Prolamin and Glutelin) in Ancient Rice Cultivars
• Antigenic Homology between Buckwheat and Wheat Prolamins
• Immunochemical Crossreactivity between Globulins from Buckwheat and Indigo Seeds
• Isolation and Molecular Cloning of a Major Wheat Allergen, Tri a Bd 27K
• Preparation and Epitope Mapping of a Monoclonal Antibody against Tri a Bd 27K, a Major Wheat Allergen
• Characterization of Inhibitors of Postprandial Hyperglycemia from the Leaves of Nerium indicum
• Effect of a Hypolipidemic Drug, Di (2-ethylhexyl) phthalate, on mRNA-Expression Associated Fatty Acid and Acetate Metabolism in Rat Tissues
• Identification of a Wheat Allergen, Tri a Bd 36K, as a Peroxidase(Food & Nutrition Science)
• Sandwich Enzyme-linked Immunosorbent Assay System for Micro-detection of the Wheat Allergen, Tri a Bd 17 K(Food & Nutrition Science)
• Immunochemical Characterization of Ovomucoid from Japanese Quail Egg White Using Monoclonal Antibodies
• Substantially Complete Removal of Three Major Allergenic Soybean Proteins (Gly m Bd 30K, Gly m Bd 28K, and the α-Subunit of Conglycinin) from Soy Protein by Using a Mutant Soybean, Tohoku 124
• Purification and Characterization of Soybean Allergen Gly m Bd 28K
• Micro-assay Method for Evaluating the Allergenicity of the Major Soybean Allergen, Gly m Bd 30K, with Mouse Antiserum and RBL-2H3 Cells
• Epitope Analysis of Soybean Major Allergen Gly m Bd 30K Recognized by the Mouse Monoclonal Antibody Using Overlapping Peptides
• Identification of the Glycosylation Site of a Major Soybean Allergen, Gly m Bd 30K
• Micro-assay to Measure the Allergenicity of a Kunitz-type Soybean Trypsin Inhibitor toward Balb/c Mice by Using RBL-2H3 Cells
• α-Subunit of β-Conglycinin, an Allergenic Protein Recognized by IgE Antibodies of Soybean-sensitive Patients with Atopic Dermatitis
• Detection of N^G, N^G-Dimethylarginine Dimethylaminohydrolase in Human Tissues Using a Monoclonal Antibody^1
• Measurement of Gly m Bd 30K, a Major Soybean Allergen, in Soybean Products by a Sandwich Enzyme-linked Immunosorbent Assay
• Identification of the Soybean Allergenic Protein, Gly m Bd 30K, with the Soybean Seed 34-kDa Oil-body-associated Protein
• Identification of a Soybean Protein Producing IgE Antibodies in BALB/c Mice
• An Isozyme of Earthworm Serine Proteases Acts on Hydrolysis of Triacylglycerol
• Comparison of Acylated Plant Pigments : Light-resistance and Radical-scavenging Ability(Biochemistry & Molecular Biology)
• Further Stabilization of Earthworm Serine Protease by Chemical Modification and Immobilization(Biochemistry & Molecular Biology)
• Thermal Rearrangement of N-Alkyl-, and N-Aryl-(2,2-dihalo-1-phenylcyclopropyl)methyleneamines to 1-Alkyl-,and 1-Aryl-2 (or 3)-halo-4-phenylpyrroles
• Investigation of Multiple Forms of Tri a Bd 27K, a Major Wheat Allergen, by Immunoblotting Analysis
• ビール中の新しい18-kDaのIgE結合蛋白質
• 3a GK-7 マークカードによる理解状態の分析
• 学問の階層性と科学教育 : 科学教育の古くて新しい哲学
• 「E=mc^2の解釈についての疑問」を読んで(談話室)
• B223 概念チャートデータベースの教育への活用
• Chart Representation of Concepts in Physics for Education
• Attenuated Listeria infection activates natural killer cell cytotoxicity to regress melanoma growth in vivo
• Differential Effect of Listeria monocytogenes Infection on Cytokine Production and Cytotoxicity of CD8 T Cells
• A two-step model of T cell subset commitment : antigen-independent commitment of T cells before encountering nominal antigen during pathogenic infections
• Molecular Cloning and Allergenicity of Pen j 1, a Major Allergen of Kuruma Prawn, Penaeus japonicus
• 科学教育についてのインフォーメイション・アナリシス
• A Pipelined Architecture for Normalized LMS Adaptive Digital Filters (Special Section on VLSI for Digital Signal Processing)
• Pipelined Architecture of the LMS Adaptive Digital Filter with the Minimum Output Latency(Special Section on Digital Signal Processing)
• Dynamical Structure Factors of Single-Walled Carbon Nanotubes by Means of Molecular Dynamics Calculation : Condensed Matter: Structure, etc.
• Probabilistic Cellular Automaton for Random Walkers : General Physics
• Effects of Acetate on Lipid Metabolism in Muscles and Adipose Tissues of Type 2 Diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats
• Improvement of Obesity and Glucose Tolerance by Acetate in Type 2 Diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats
• Modulation of the Immune System by Listeria monocytogenes-Mediated Gene Transfer into Mammalian Cells
• A Novel Type of Two-Component Regulatory System Affecting Gingipains in Porphyromonas gingivalis
• Preparation of Hypoallergenic Soybean Protein with Processing Functionality by Selective Enzymatic Hydrolysis
• Codeblock-Based Error Concealment for JPEG2000 Coded Image Transmission over RTP(Digital Signal Processing)
• QOS ESTIMATION BASED ON PSNR FOR MOTION JPEG2000 VIDEO IN LOSSY PACKET NETWORKS(International Workshop on Advanced Image Technology 2007)
• QOS ESTIMATION BASED ON PSNR FOR MOTION JPEG2000 VIDEO IN LOSSY PACKET NETWORKS
• Codeblock-Based Error Concealment for JPEG 2000 Coded Image Transmission over Lossy Packet Networks(音響信号処理/一般)
• Codeblock-Based Error Concealment for JPEG 2000 Coded Image Transmission over Lossy Packet Networks
• Codeblock-Based Error Concealment for JPEG 2000 Coded Image Transmission over Lossy Packet Networks
• LOW DELAY PRE-FEC INTERLEAVING SCHEME FOR ROBUST JPEG2000 VIDEO STREAMING IN PACKET LOSS ENVIRONMENT(International Workshop on Advanced Image Technology 2006)
• Error Protection for JPEG2000-Coded Images and Its Evaluation over OFDM Channel(Digital Signal Processing)
• Investigation of Multiple Forms of Tri a Bd 27K, a Major Wheat Allergen, by Immunoblotting Analysis
• Tumor-Suppressive Lipoxygenases Inhibit the Expression of c-myc mRNA Coding Region Determinant-Binding Protein/Insulin-Like Growth Factor II mRNA-Binding Protein 1 in Human Prostate Carcinoma PC-3 Cells
• Effects of Acetate on Lipid Metabolism in Muscles and Adipose Tissues of Type 2 Diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats
• Preparation and Epitope Mapping of a Monoclonal Antibody against Tri a Bd 27K, a Maior Wheat Allergen
• Molecular Cloning and Allergenicity of Pen j 1, a Major Allergen of Kuruma Prawn, Penaeus japonicus
• Isolation and Molecular Cloning of a Major Wheat Allergen, Tri a Bd 27K
• ビール中の新しい18-kDaのIgE結合蛋白質
• Improvement of Obesity and Glucose Tolerance by Acetate in Type 2 Diabetic Otsuka Long-Evans Tokushima Fatty (OLETF) Rats
• Effect of a Hypolipidemic Drug, Di (2-ethylhexyl) phthalate, on mRNA-Expression Associated Fatty Acid and Acetate Metabolism in Rat Tissues
• Identification of a Two-Component Signal Transduction System Involved in Fimbriation of Porphyromonas gingivalis
• 2p-N-6 分子拡散実験の指導について
• Gastrointestinal Digestion and Absorption of Pen j 1, a Major Allergen from Kuruma Prawn, Penaeus japonicus
• Quantitative Analysis of Gly m Bd 28K in Soybean Products by a Sandwich Enzyme-Linked Immunosorbent Assay
• Purification and Partial Characterization of Acetyl-CoA Synthetase in Rat Liver Mitochondria
• Analysis and Functionality of Major Polyphenolic Components of Polygonum cuspidatum (Itadori)
• 29p-AB-5 パルス光による光速の測定実験(物理教育,第41回年会)

もっと見る 閉じる

スポンサーリンク