Identification of the Soybean Allergenic Protein, Gly m Bd 30K, with the Soybean Seed 34-kDa Oil-body-associated Protein
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概要
- 論文の詳細を見る
The soybean allergenic protein, Gly m Bd 30K [Ogawa et al., J. Nutr. Sci. Vitamihol., 37, 555-565(1991)] which is most strongly and frequently recognized by the IgE antibodies in sera of soybean-sensitive patients with atopic dermatitis, has been characterized. The allergen was isolated from the crude 7S-globulin fraction as an oligomeric form with a molecular weight of more than 3000,000 by gel-filtration chromatography. On two-dimensional gel electrophoresis, the native oligomeric allergen had an isoelectric point of about pH 4.5 and was dissociated into a monomeric form with a molecular weight of about 32,000 by the treatment with sodium dodecyl sulfate and 2-mercaptoethanol. The monomeric allergen had an N-terminal amino acid sequence and amino acid composition identical with those of the soybean seed 34-kDa oil-body-associated protein or the soybean vacuolar protein P34 with close homology to papain-like thiol proteinases [Kalinski et al., J. Biol. Chem., 267, 12068 (1992)]. The identity was further confirmed by the immunological cross-reactivity to the antibodies produced against each of the purified allargen and the 34-kDa oil-body-associated protein. By this observation, Gly m Bd 30K was shown to have about 30% sequence homology with Der pI, a house dust mite allergen that is a thiol proteinase from Dermatophagoides pteronyssius.
- 社団法人日本農芸化学会の論文
- 1993-06-23
著者
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NISHIKAWA Kiyoshi
Department of Pediatrics, National Kagawa Children's Hospital
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西川 喜良
甲南大学理学部
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TSUJI Hideaki
Department of Nutritional Science, Okayama Prefectural University
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Hirano Hisashi
National Institute Of Agrobiological Resources Ministry Of Agriculture Forestry And Fisheries
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Hirano Hisashi
Department of Molecular Biology, National Institute of Agrobiological Resources
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Tsuji Hiroaki
Nisshin Oil Mills Ltd.
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Ogawa T
Kyoto Univ. Kyoto Jpn
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Kitamura Keiko
Institute Of Applied Biochemistry University Of Tsukuba
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Ogawa Takahiro
Department Of Biological Science And Technology Science University Of Tokyo
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Tsuji H
Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.
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Tsuji Hideaki
Graduate School Of Health And Welfare Science Okayama Prefectural University
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Tsuji Hideaki
Department Of Chemistry Faculty Of Education Gifu University
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Hirano H
National Inst. Agrobiological Res. Tsukuba Jpn
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Hirano Hisashi
Department Of Molecular Biology National Institute Of Agrobiological Resources
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BANDO Noriko
Department of Nutrition, School of Medicine, The University of Tokushima
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OGAWA Tadashi
Department of Nutrition, School of Medicine, The University of Tokushima
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Kitamura Keisuke
Legume Breeding Laboratory, National Agriculture Research Center
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Zhu Yue-Lin
Legume Breeding Laboratory, National Agriculture Research Center
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Tsuji Hideaki
Department Of Nutritional Science Okayama Prefectural University
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Ogawa Takahira
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Ogawa Takahira
Dept. Appl. Microbial Technology Kumamoto Institute Of Technology
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Kitamura Keisuke
Legume Breeding Laboratory National Agriculture Research Center
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Nishikawa Kiyoshi
Department Of Electrical Engineering Tokyo Metropolitan University
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Nishikawa K
Department Of Pediatrics National Kagawa Children's Hospital
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Nishikawa K
Course. Of Info. And Commn. Sys. Eng. Tokyo Metro. Univ.
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Ogawa Tadashi
Department Of Legal Medicine Aichi Medical University School Of Medicine
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Zhu Yue-lin
Legume Breeding Laboratory National Agriculture Research Center
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Noriko Bando
Department Of Food Science Graduate School Of Nutrition And Biosciences The University Of Tokushima
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Kitamura K
Ministry Of Agriculture Forestry And Fisheries Tokyo Jpn
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Bando Noriko
Department Of Food Science Graduate School Of Nutrition And Bioscience The University Of Tokushima
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Nishikawa Kiyoshi
Department Of Chemistry Faculty Of Science Kanazawa University
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Ogawa Tadashi
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University
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