Preparation and Epitope Mapping of a Monoclonal Antibody against Tri a Bd 27K, a Major Wheat Allergen

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概要

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• We produced a monoclonal antibody (mAb) as a probe for detection of Tri a Bd 27K, a major wheat allergen. The mAb recognized the allergen purified from wheat flour, and the epitope on the allergen to the mAb was determined to be amino acid sequence 154VPWVVVDGKPL164 of Tri a Bd 27K. Of the amino acid residues on the epitope, the amino acid residues responsible for the binding to the mAb were found to be W156, D160, G161, and P163.

• 社団法人 日本農芸化学会の論文

著者

• HIEMORI Miki

  Department of Nutritional Science, Okayama Prefectural University

• YAMASHITA Hiromi

  Department of Nutritional Science, Okayama Prefectural University

• TSUJI Hideaki

  Department of Nutritional Science, Okayama Prefectural University

• SUZUKI Makiko

  Department of Hematology and Rheumatology, Tohoku University Hospital

• Kimoto Masumi

  Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University

• Komiyama Nobuko

  Department of Human Nutrition, Faculty of Contemporary Life Science, Chugoku Gakuen University

• Komiyama Nobuko

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Kimoto Masumi

  Department Of Nutritional Science Okayama Prefectural University

• TAKAHASHI Yoshitaka

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural Univer

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