Development of an Enzyme-Linked Immunosorbent Assay System for the Determination of Asymmetric Dimethylarginine Using a Specific Monoclonal Antibody

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概要

• 論文の詳細を見る

• We produced a monoclonal antibody (mAb) against <I>N</I><SUP>G</SUP>,<I>N</I><SUP>G</SUP>-dimethyl-L-arginine (asymmetric dimethylarginine: ADMA), an endogenous competitive inhibitor of nitric oxide synthase (NOS), and developed an enzyme-linked immunosorbent assay (ELISA). The competitive ELISA method using the mAb determined 5 nM–100 nM ADMA, and ADMA levels in human plasma and urine were found to be 0.78 μM and 51.3 μmol/g of creatinine respectively.

• Japan Society for Bioscience, Biotechnology, and Agrochemistryの論文
• 2012-02-23

著者

• Suzuki Makiko

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Kimoto Masumi

  Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University

• Suzuki Makiko

  Department Of Hematology And Rheumatology Tohoku University Hospital

• Tsuji Hiroaki

  Nisshin Oil Mills Ltd.

• Tsuji H

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Tsuji Hideaki

  Graduate School Of Health And Welfare Science Okayama Prefectural University

• Tsuji Hideaki

  Department Of Chemistry Faculty Of Education Gifu University

• Tsuji Hideaki

  Department Of Nutritional Science Okayama Prefectural University

• Yamasita Hiromi

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Yamato Makiko

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Takahashi Y

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Yamashita Hiromi

  Department Of Applied Chemistry College Of Engineering Osaka Prefecture University

• Kimoto M

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Yokoro Miyuki

  Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi

• Takahashi Yoshitaka

  Department Of Applied Physics Faculty Of Engineering University Of Tokyo

• YATANI Machiko

  Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University

• Kimoto Masumi

  Department of Immunology and Host Defenses, Ehime University Graduate School of Medicine

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