Dioscorea japonica extract down-regulates prostaglandin E2 synthetic pathway and induces apoptosis in lung cancer cells
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概要
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Prostaglandin E2 plays a role in an array of pathophysiological responses, including inflammation, carcinogenesis and so on. Prostaglandin E2 is synthesized from arachidonic acid by the enzymes cyclooxygenase and prostaglandin E synthase. In some pathological conditions, the isozymes cyclooxygenase-2 and microsomal prostaglandin E synthase-1 are transiently induced, leading to prostaglandin E2 overproduction. The present study showed that Dioscorea japonica extract suppresses mRNA expression of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 in human non-small-cell lung carcinoma A549 cells in a dose-dependent manner. The suppressive effects of Dioscorea japonica extract on the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase-1 were confirmed by Western blotting, cyclooxygenase activity and prostaglandin E2 production. Dioscorea japonica extract induced the translocation of nuclear factor-κB from the nucleus to the cytosol and inhibited the activity of the cyclooxygenase-2 promoter. Furthermore Dioscorea japonica extract suppressed the expression of the anti-apoptotic factor B-cell chronic lymphocytic leukemia/lymphoma 2 and enhanced apoptotic terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive intensity in A549 cells. These results suggest that Dioscorea japonica extract suppresses the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase-1, with the regulation of the transcriptional activity of cyclooxygenase-2, and induces apoptosis in cancer cells. Thus, Dioscorea japonica may contribute to the prevention of prostaglandin E2-mediated pathophysiological responses such as carcinogenesis and inflammation.
- 日本酸化ストレス学会 JCBN事務局の論文
著者
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Tanaka Sayuri
Department Of Pathology Tohoku University School Of Medicine
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Kawakami Yuki
Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Universi
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Takahashi Yoshitaka
Department Of Applied Physics Faculty Of Engineering University Of Tokyo
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Kimoto Masumi
Department of Immunology and Host Defenses, Ehime University Graduate School of Medicine
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Arakawa Toshiya
Department of Biochemistry, School of Dentistry, Health Sciences University of Hokkaido
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Suzuki-Yamamoto Toshiko
Department of Nutritional Science, Okayama Prefectural University
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Tsukayama Izumi
Department of Nutritional Science, Okayama Prefectural University
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Takafuji Miki
Department of Nutritional Science, Okayama Prefectural University
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Hanada Takae
Department of Nutritional Science, Okayama Prefectural University
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