Preparation and Application of Monoclonal Antibodies for a Sandwich Enzyme-Linked Immunosorbent Assay of the Major Soybean Allergen, Gly m Bd 30K.
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概要
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In order to obtain probes suitable for the determination of the major soybean allergen, Gly m Bd 30 K, in soybean-related processed foods, we have prepared two monoclonal antibodies, F5 of IgG2a and H6 of IgM, by the fusion of P3U1 myeloma cells with the spleen cells of BALB/c mice immunized with the reductively carboxymethylated aller-gen (RCM-allergen). The two monoclonal antibodies were shown to be specific to the intact allergen as well as the RCM-allergen and to recognize distinct epitopes on the allergen. Of the monoclonal antibodies, F5 was conjugated with peroxidase. H6 and the labeled F5 were applied as the fixing antibody and the labeled first antibody, respectively, for a direct sandwich enzyme-linked immunosorbent assay of the allergen. When the allergen was extracted from the soybean-related foods with Tris-HCl buffer containing sodium dodecylsulfate and mercaptoethanol, the aller-gen, Gly m Bd 30 K, was shown to be measured in a range of 5-500 ng in this assay.
- 財団法人 学会誌刊行センターの論文
著者
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Tsuji Hideaki
Department Of Chemistry Faculty Of Education Gifu University
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Bando Noriko
Department Of Food Science Graduate School Of Nutrition And Bioscience The University Of Tokushima
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Okada Naoko
Department Of Biochemistry Nihon University School Of Medicine
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Kimoto Masumi
Department of Immunology and Host Defenses, Ehime University Graduate School of Medicine
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Ogawa Tadashi
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University
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