Micro-assay to Measure the Allergenicity of a Kunitz-type Soybean Trypsin Inhibitor toward Balb/c Mice by Using RBL-2H3 Cells

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概要

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• The allergenicity of a Kunitz-type soybean trypsin inhibitor (KSTI) was investigated by a micro-assay of β-N-acetylhexosaminidase released from RBL-2H3 cells primed with the anti-KSTI serum. KSTI stimulated the release of β-N-acetylhexosaminidase from RBL-2H3 cells primed with the antiserum. The response of RBL-2H3 cells to the reaginic activity of the mouse anti-KSTI serum correlates fairly well with that by the passive cutaneous anaphylaxis (PCA) test, the sensitivity of both assays appearing to be similar. These results suggest that measuring the β-N-acetylhexosaminidase released from RBL-2H3 is a convenient way for studying the allergen or the reaginic activity of a murine serum in place of the PCA test.

• 社団法人日本農芸化学会の論文
• 1995-07-23

著者

• TSUJI Hideaki

  Department of Nutritional Science, Okayama Prefectural University

• Tsuji Hiroaki

  Nisshin Oil Mills Ltd.

• Ogawa T

  Kyoto Univ. Kyoto Jpn

• Ogawa Takahiro

  Department Of Biological Science And Technology Science University Of Tokyo

• Tsuji H

  Dep. Of Nutritional Sci. Fac. Of Health And Welfare Sci. Okayama Prefectural Univ.

• Tsuji Hideaki

  Graduate School Of Health And Welfare Science Okayama Prefectural University

• Tsuji Hideaki

  Department Of Chemistry Faculty Of Education Gifu University

• OGAWA Tadashi

  Department of Nutrition, School of Medicine, The University of Tokushima

• YAMANISHI Rintaro

  Department of Nutrition, School of Medicine, The University of Tokushima

• Kondo Kiwako

  Department of Nutrition, School of Medicine, The University of Tokushima

• Tsuji Hideaki

  Department Of Nutritional Science Okayama Prefectural University

• Ogawa Takahira

  Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University

• Ogawa Takahira

  Dept. Appl. Microbial Technology Kumamoto Institute Of Technology

• Ogawa Tadashi

  Department Of Legal Medicine Aichi Medical University School Of Medicine

• Kondo Kiwako

  Department Of Nutrition School Of Medicine The University Of Tokushima

• Yamanishi Rintaro

  Department Of Food Science Graduate School Of Nutrition And Biosciences The University Of Tokushima

• Yamanishi Rintaro

  Laboratory Of Nutritonal Chemistry Department Of Food Science And Technology Faculty Of Agriculture

• Ogawa Tadashi

  Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo University

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