Ethylene Production Using a Broad-Host-Range Plasmid in Pseudomonas syringae and Pseudomonas putida
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概要
- 論文の詳細を見る
We have constructed a high-copy-number, broad-host-range plasmid, pMEFE1 that carries a gene encoding an ethylene-forming enzyme from Pseudomonas syringae pv. phaseolicola PK2. The ethylene-forming enzyme was expressed under the control of a promoter of the neomycin phosphotransferase gene on pMEFE1. When introduced into P. syringae and Pseudomonas putida cells by transconjugation, pMEFE1 conferred on the recombinant cells the ability to synthesize the ethylene-forming enzyme with specific activities 41 and 20 times higher, respectively, than that of the ethylene-forming enzyme in the parental P. syringae.
- 社団法人日本生物工学会の論文
- 1996-11-25
著者
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MATSUOKA MASAYOSHI
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo Universi
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OGAWA TAKAHIRA
Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo Universi
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Ogawa T
Shinshu Univ. Nagano Jpn
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Fukuda H
Kobe Univ. Kobe
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Fukuda Hideki
Department Of Molecular Science And Material Engineering Graduate School Of Science And Technology K
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Ogawa T
Kyoto Univ. Kyoto Jpn
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Ogawa Takahiro
Department Of Biological Science And Technology Science University Of Tokyo
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Ogawa T
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Matsuoka M
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Matsuoka Masayoshi
Dept. Applied Microb. Technol. Sojo Univ.
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FUKUDA HIDEO
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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ISHIHARA KATSUYA
Department of Applied Microbial Technology, Kumamoto Institute of Technology
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Fukuda H
Kobe Univ. Kobe Jpn
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Ogawa T
Kumamoto Inst. Technol. Kumamoto Jpn
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Ogawa Takahira
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Ogawa Takahira
Dept. Appl. Microbial Technology Kumamoto Institute Of Technology
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Matsuoka Masayoshi
Dept. Appl. Microbial Technology Kumamoto Institute Of Technology
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Matsuoka Masayoshi
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Fukuda Hideo
Department Of Applied Microbial Technology The Kumamoto Institute Of Technolgy
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Fukuda Hideo
Dept. Appl. Microbial Technology Kumamoto Institute Of Technology
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Ogawa Tadashi
Graduate School Of Bioagricultural Sciences Nagoya University
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Ishihara Katsuya
Division Of Cardiology And Cardiac Surgery Kofu Johnan Hospital
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Fukuda Hideo
Department Of Applied Microbial Technology Kumamoto Institute Of Technology
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