Functional replacement of yeast flavocytochrome b_2 with bacterial L-lactate dehydrogenase(GENETICS, MOLECULAR BIOLOGY, AND GENE ENGINEERING)

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概要

• 論文の詳細を見る

• Using yeast genetic complementation, we show here that Rhodobacter sphaeroides L-lactate dehydrogenase can functionally replace flavocytochrome b_2 in Saccharomyces cerevisiae, only when a matrix-targeting signal of flavocytochrome b_2 is fused with the bacterial enzyme. The recombinant L-lactate dehydrogenase may add alternative route of mitochondrial electron transport other than flavocytochrome b_2.

著者

• Morimura Shigeru

  Graduate School of Science and Technology, Kumamoto University

• Kida Kenji

  Graduate School of Science and Technology, Kumamoto University

• Morimura Shigeru

  Graduate School Of Science And Technology Kumamoto University

• NAGAHAMA KAZUHIRO

  Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo Universi

• MATSUOKA MASAYOSHI

  Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo Universi

• Sakai Hiroaki

  Department Of Thoracic Surgery Kyoto University Hospital

• 中村 薫

  京大 化研

• 中村 厚三

  Univ. Tokyo Tokyo Jpn

• Taguchi Hisataka

  Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo Universi

• Akamatsu Takashi

  Department of Applied Microbial Technology, Faculty of Biotechnology and Life Science, Sojo Universi

• Matsuoka M

  Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University

• Matsuoka Masayoshi

  Dept. Applied Microb. Technol. Sojo Univ.

• Nagahama Kazuhiro

  Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University

• Kida Kenji

  Graduate School Of Science And Technology Kumamoto University

• Sakai Hiroaki

  Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University

• Nagahama Kazuhiro

  Department Of Applied Microbial Technology The Kumanoto Institute Of Technology

• Matsuoka Masayoshi

  Dept. Appl. Microbial Technology Kumamoto Institute Of Technology

• Matsuoka Masayoshi

  Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University

• Akamatsu Takashi

  Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University

• Sakai Hiroaki

  Department Of Anesthesiology Juntendo University Shizuoka Hospital

• Morimura Shigeru

  Graduate School Of Science And Technology Department Of Materials And Life Science Kumamoto Universi

• Taguchi Hisataka

  Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University

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