The Rapid Degradation of Mutant SecA Protein in the Bacillus subtilis secA341 (ts) Mutant Causes a Protein Translocation Defect in the Cell
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概要
- 論文の詳細を見る
To study the function of SecA protein and the protein translocation system of Bacillus subtilis, wild-type and mutant SecA proteins were characterized in vivo and in vitro. SecA protein was abundant in a wild-type strain (168) and existed in a stable homodimer. In contrast to this, SecA341 (ts) protein having an amino acid replacement from proline to leucine at residue 431 was undetectable by immunoblotting in the cell lysate of a secA341 mutant (TB301) at the nonpermissive temperature, 42℃. Pulse-chase studies using ^<35>S-methionine showed that newly synthesized SecA protein was rapidly degraded in the mutant at 42℃. Purified SecA341 protein was more sensitive to trypsin and subtilisin than purified wild-type SecA protein in the presence of ATP. These results indicate that the secA341 mutation causes the rapid degradation of mutant SecA protein and a concomitant protein translocation defect in the cell.
- 社団法人日本農芸化学会の論文
- 1994-10-23
著者
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中村 薫
京大 化研
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YAMANE Kunio
Institute of Biological Sciences, University of Tsukuba
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Yamane K
Univ. Tsukuba Ibaragi Jpn
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Yamane K
Institute Of Biological Sciences University Of Tsukuba
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Yamane Kunio
Institute Of Biological Sciences University Of Tsukuba
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TAKAMATSU Hiromu
Faculty of Pharmaceutical Sciences, Setsunan University
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NAKAMURA Kouji
Institute of Biological Sciences, University of Tsukuba
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Nagahama Kazuhiro
Department Of Applied Microbial Technology The Kumanoto Institute Of Technology
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Takamatsu Hiromu
Institute of Biological Sciences, University of Tsukuba
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Nakane Akitaka
Institute of Biological Sciences, University of Tsukuba
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Sadaie Yoshito
National Institute of Genetics
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Sadaie Y
Area Of Biochemistry And Molecular Biology Div. Of Life Sci. Graduate School Of Sci. And Engineering
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Nakane Akitaka
Institute Of Biological Sciences University Of Tsukuba
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Nakamura Kahoru
Department Of Nutrition Tokyo University Of Agriculture
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Sadaie Y
Area Of Biochemistry And Molecular Biology Div. Of Life Sci. Graduate School Of Sci. And Engineering
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Takamatsu H
Fac. Of Pharmaceutical Sciences Setsunan Univ.
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Nakamura Kouji
Institute Of Biological Sciences University Of Tukuba
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Nakamura Kouji
Institute Of Biological Sciences University Of Tsukuba
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YAMANE Kunio
Institute of Applied Microbiology, The University of Tokyo
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