Multi-enzymatic Glucosylation Using Eucalyptus UDP-Glucosyltransferase Coupled UDPglucose-Fermentation by Bakers'Yeast
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概要
- 論文の詳細を見る
The enzymatic synthesis of glucoside compounds using a membrane-associated UDP-glucosyltransferase fraction from Eucalyptus perriniana cultured cells as a water-insoluble catalyst (N. Nakajima, et. al., J. Ferment. Bioeng., 84 (5), pp. 455-460,1997) has been effectively done by coupling UDPglucose-fermentation by bakers' yeast. Forexample, β-thujaplicin (hinokitiol) and p-aminobenzoic acid were converted respectively to their corresponding β-D-monoglucosides with the conversion rate of around 24-26% by the multi-enzymatic system with UDPglucose as a glucose donor, which is produced by yeast cells from glucose and 5'-UMP. Addition of either cellobiose, a substrate of β-glucosidase, or DL-1,2-anhydro-myo-inositol, an inhibitor for the enzyme in the reaction mixture, could increased the yield of these β-D-monoglucosides. This new enzymatic system could also be used for the synthesis of flavonoid glucosides such as isoquercitrin (quercetin 3-O-β-D-glucoside).
- 社団法人日本農芸化学会の論文
- 1999-05-23
著者
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中村 薫
京都大学化学研究所
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中島 伸佳
Graduate School Of Health And Welfare Okayama Prefectural University
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中島 伸佳
岡山県立大学短期大学部
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中島 伸佳
岡山県立短大
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NAKAJIMA Nobuyoshi
Department of Nutritional Science, Okayama Prefectural University
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ISHIHARA Kohji
Department of Chemistry, Kyoto University of Education
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NAKAMURA Kaoru
Institute for Chemical Research, Kyoto University
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HAMADA Hiroki
Department of Applied Science, Okayama University of Science
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YAMANE Shinya
Department of Applied Science, Okayama University of Science
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FURUYA Tsutomu
Department of Applied Science, Okayama University of Science
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Hamada H
National Institute Of Fitness And Sports In Kanoya
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中村 薫
京大 化研
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Ishihara K
Dep. Of Life Sci. Okayama Univ. Of Sci. 1-1 Ridai-cho Kita-ku Okayama 700-0005 Jpn
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Nakajima Nobuyoshi
Graduate School Of Health And Welfare Okayama Prefectural University
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Nakajima Nobuyoshi
Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University
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Nagahama Kazuhiro
Department Of Applied Microbial Technology The Kumanoto Institute Of Technology
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Nakamura Kaoru
Institute For Chemical Research Kyoto University
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Furuya Tsutomu
School Of Pharmaceutical Sciences Kitasato University
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Furuya Tsutomu
Department Of Applied Sci. Okayama University Of Science
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Yamane Shinya
Department Of Applied Science Okayama University Of Science
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Nakamura Kahoru
Department Of Nutrition Tokyo University Of Agriculture
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Hamada Hiroki
Department Of Applied Sci. Okayama University Of Science
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Furuya Tsutomu
Department of Applied Sci. , Okayama University of Science
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Nakajima Nobuyoshi
Department of Food and Nutrition, Okayama Prefectural Junior College
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HAMADA Hiroki
Department of Applied Sci. , Okayama University of Science
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