Chemical Modification of Earthworm Fibrinolytic Enzyme with Human Serum Albumin Fragment and Characterization of the Protease as a Therapeutic Enzyme
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概要
- 論文の詳細を見る
The strongest tibrinolytic protease (F-III-2) in the six enzyme proteins purified from earthworm, Lumbricus rubellus [N. Nakajima et al., Biosci. Biotech. Biochem., 57, 1726-1730 (1993)] has been modified chemically with fragmented human serum albumin (mol. wt., 10,000-30,000). The modified enzyme lost the antigenicity of the native enzyme and reacted with the antisera against human serum albumin, the human serum albumin fragments, and the conjugate with the natiVe enzyme to form precipitation lines, which fused with each other. The conjugate was significantly more resistant to inactivation by protease inhibitors in rat plasma. The enzyme was a non-hemorrhagic protein and did not induce platelet aggregation. The enzyme kept potent proteolytic activity for fibrin and fibrinogen than that of human plasmin. The enzyme easily solubilized actual fibrin clots (thrombi) of whole blood induced by thrombin in a rat's vena cava. The continuous fibrinolysis for fibrin suspension in an enzyme reactor system using the modified enzyme immobilized to oxirane-activated acrylic beads has been achieved without any inactivation of the activity at least for more than 1 month. The N-terminal amino acid sequence of the protein was also investigated and the sequence showed local similarity to those of the serine proteases such as plasmin and chymotrypsin.
- 社団法人日本農芸化学会の論文
- 1996-02-23
著者
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中島 伸佳
岡山県立大学短期大学部
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中島 伸佳
岡山県立短大
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NAKAJIMA Nobuyoshi
Department of Nutritional Science, Okayama Prefectural University
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SUGIMOTO Manabu
Research Institute for Bioresources, Okayama University
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ISHIHARA Kohji
Department of Chemistry, Kyoto University of Education
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HAMADA Hiroki
Department of Applied Science, Okayama University of Science
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Hamada H
National Institute Of Fitness And Sports In Kanoya
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Ishihara K
Dep. Of Life Sci. Okayama Univ. Of Sci. 1-1 Ridai-cho Kita-ku Okayama 700-0005 Jpn
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SUMI Hiroyuki
Department of Physiological Chemistry, College of Science & Industrial Technology, Kurashiki Univers
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MIKUNI Katsuhiko
Bio Research Corporation of Yokohama
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Nakajima Nobuyoshi
Graduate School Of Health And Welfare Okayama Prefectural University
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Nakajima Nobuyoshi
Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University
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MIKUNI KATSUHIKO
Ensuiko Sugar Refining Co., Ltd.,
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Mikuni K
Bio Research Corporation Of Yokohama
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Mikuni K
Carbohydrate Research Laboratory Ensuiko Sugar Refining Co. Ltd.
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Mikuni Katsuhiko
Ensuiko Sugar Refining Co. Ltd.
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Sugimoto M
Research Institute For Bioresources Okayama University
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Sugimoto Manabu
Research Institute For Bioresources Okayama University
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Sumi Hiroyuki
Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Univ.
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Hamada Hiroki
Department Of Applied Sci. Okayama University Of Science
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Sugimoto Manabu
Research Instiitte For Bioresources Okayama
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SUMI Hiroyuki
Department of Physiological Chemistry, Kurashiki University of Science and the Arts
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Nakajima Nobuyoshi
Department of Food and Nutrition, Okayama Prefectural Junior College
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HAMADA Hiroki
Department of Applied Sci. , Okayama University of Science
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