Multiple Molecular Forms of α-Glucosidase from Spinach Seeds, Spinacia oleracea L.
スポンサーリンク
概要
- 論文の詳細を見る
Four molecular forms of α-glucosidase were isolated from spinach seeds by several kinds of chromatography. The molecular masses of α-glucosidases I, II, III, and IV were 78, 78, 82, and 82kDa by SDS-PAGE, and 62, 62, 190, and 70kDa by gel filtration, respectively. α-Glucosidases I and II showed similar enzymatic properties, in which the K_m for soluble starch was about 10 times lower than that for maltose, and they had higher activity not only toward malto-oliosaccharides but also toward α-glucans. The optimum pH was 4.5-5.5 and about 50% of the activity remained after incubation at 70℃ for 20min. On the other hand, α-glucosidases III and IV showed similar enzymatic properties, in which the K_m for maltose was 3-4 times lower than that for soluble starch, and they had high activity toward malto-oligosaccharides but faint activity toward α-glucans. The optimum pH was 4.5-5.0 and no activity was found after incubation at 70℃ for 20min. However, anti-α-glucosidase III serum formed precipitation specifically with α-glucosidase III.
- 社団法人日本農芸化学会の論文
- 1995-04-23
著者
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SUGIMOTO Manabu
Research Institute for Bioresources, Okayama University
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Suzuki Yoshihito
Dep. Of Applied Biological Chemistry Univ. Of Tokyo
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SUZUKI Yukio
Research Institute for Bioresources, Okayama University
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Suzuki Y
Department Of Global Epidemiology Hokkaido University Research Center For Zoonosis Control
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Suzuki Yoshihito
Department Of Applied Biological Chemistry The University Of Tokyo
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Suzuki Yoshihito
The Institute Of Enology And Viticulture Yamanashi University
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Sugimoto M
Research Institute For Bioresources Okayama University
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Sugimoto Manabu
Research Institute For Bioresources Okayama University
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Suzuki Y
Research Institute For Bioresources Okayama University
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FURUI Satoshi
Research Institute for Bioresources, Okayama University
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Furui Satoshi
Research Institute For Bioresources Okayama University:(present Address)nippon Gene Co. Ltd.
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Suzuki Yukio
Research Institute For Bioresources Okayama University
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Suzuki Yukio
Research Insititute For Bioresources Okayama University
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Sugimoto Manabu
Research Instiitte For Bioresources Okayama
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