Transphosphatidylation Reactioin of Phosphatidylcholine to 4-Methoxyphyenol in Water-Immiscible Organic Solvents with Immobilized Phospholipase D
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概要
- 論文の詳細を見る
Immobilized phospholipase D (PLD) from Streptomyces sp. catalyzed the transfer reaction of the dipalmitoylphosphatidyl residue from 1,2-dipalmitoryl-3-sn-phosphatidylcholine (DPPC) to an aromatic hydroxy group on 4-methoxyphenol in water-immiscible organic solvents, to afford 1,2-dipalmitoyl-3-sn-phosphatidyl-4-methoxyphenol (DPP-PMP) with a 45% yield, accompanied by a trace amount of 1,2-dipalmitoyl-3-sn-phosphatidic acid sodium salt (DPPA-Na). The formation of DPP-PMP was affected by organic solvents used in the reaction. Benzene, toluene, and methylene chloride gave DPP-PMP with moderate yields but use of diethyl ether resulted in a low yield of DPP-PMP. In both ethyl acetate and water-miscible organic solvents, the transfer reaction did not take place. Immobilization of PLD was carried out by adding a 1% volume of PLD solution to a suspension of a catioin-exchange resin (Amberlite ICR-50,5% w/v) in benzene with stirring and sonication. In a repeated batch reaction of DPP-PMP synthesis with immobilized PLD, after ten batch cycles the enzyme retained 74% of its initial activity.
- 公益社団法人日本生物工学会の論文
- 1995-04-25
著者
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SUZUKI Yukio
Research Institute for Bioresources, Okayama University
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Takami Masaaki
Research Institute For Bioresources Okayama University
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Suzuki Y
Tohoku Univ. School Of Medicine Sendai Jpn
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Suzuki Yukio
Research Institute For Bioresources Okayama University
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Suzuki Yukio
Research Insititute For Bioresources Okayama University
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