Subsite Affinities of α-Glucosidase from Mucor javanicus
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概要
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The rate parameters for maltooligosaccharides of α-glucosidase from Mucor javanicus were studied. The K_m and k_o values (mM, s^<-1>) for maltose, malto-triose, -tetraose, -pentaose, -hexaose, and -heptaose were estimated to be (0.60, 334); (0.29, 271); (0.27, 312); (0.23, 285); (0.14, 230); (0.11, 184) in this order. The subsite affinities in the active site were 1.78, 5.31, 0.317, 0.126, 0.039, 0.168, and 0.002 kcal/mol for subsites 1, 2, 3, 4, 5, 6, and 7, respectively. From these results, it is considered that extended subsites exist in the enzyme, where maltooligosaccharides could be bound.
- 社団法人日本農芸化学会の論文
- 1993-08-23
著者
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SUGIMOTO Manabu
Research Institute for Bioresources, Okayama University
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SUZUKI Yukio
Research Institute for Bioresources, Okayama University
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Sugimoto Manabu
Research Institute For Bioresources Okayama University
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Suzuki Yukio
Research Institute For Bioresources Okayama University
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Suzuki Yukio
Research Insititute For Bioresources Okayama University
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Sugimoto Manabu
Research Instiitte For Bioresources Okayama
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