Comparative Study of Pyridoxine-α, β-Glucosides, and Phosphopyridoxyl-Lysine as a Vitamin B6 Nutrient

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The nutritional effects of pyridoxine-a-glucoside (PN-α-Glc), pyridoxine-5β-glucoside (PN-5β-Glc) and ε-N-(phosphopyridoxyl)-lysine (PNP-Lys) were examined by means of: 1) transport across the intestinal wall using everted rat intestine, 2) metabolic conversion by liver or kidney homogenate, and 3) oral administration of the compound to B6-deficient rats and the subsequent analysis of B6 derivatives found in plasma. Using everted sacs prepared from rat small intestine, PN-α-Glc was transported into the serosal side in its intact form. On the other hand, a part of PN-5β-Glc was found as PN on the serosal side (PN-5β-Glc: PN=2:1) . When PN-α-Glc, PN-5β-Glc or PNP-Lys was incubated with liver or kidney homogenate for 3h at pH 6.0, PN-α-Glc was hydrolyzed to PN (6%), while there was no hydrolysis of PN-5β-Glc. After 30min of administration of each B6 derivative to B6-deficient rats, blood was collected from the heart, and the B6-derivatives found in plasma were analyzed. It was ascertained that PN-α-Glc served as well as PN as a B6 nutrient, while PN-5β-Glc and PNP-Lys were not easily metabolized to the coenzyme form, pyridoxal 5-phosphate.