Fluorescence Enhancement of PLP for Application to HPLC(Food & Nutrition)
スポンサーリンク
概要
- 論文の詳細を見る
In an HPLC analysis of vitamin B6 derivatives, the use of a reversed-phase ODS column in conjunction with a fluorescence detector has given promising results. However, PLP was rather insensitive to the analysis, making it difficult to obtain accurate results, especially in biological materials. Subsequently, a pre-column treatment that involved extracting the sample solution with 1 M perchloric acid, heating the extract for 3 hr at 50°C in the presence of 5mM KCN at pH 7.5, and then allowing it to stand for 24hr at room temperature served to overcome difficulties in the analysis. The fluorescence enhancement of PLP was over 10-fold and produced a single peak that was applicable to the quantification of PLP in biological materials.
- 社団法人日本農芸化学会の論文
- 1988-04-23
著者
-
Shoji Takao
Department Of Agricultural Chemistry Faculty Of Agriculture Gifu University
-
Suda Hiroyuki
Nagoya Branch Of Japan Food Research Laboratories
-
Tsuge Haruhito
Department Of Agricultural Chemistry Faculty Of Agriculture Gifu University
-
TOUKAIRIN-ODA Toshikatu
Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University
-
SAKAMOTO Eiko
Department of Agricultural Chemistry, Faculty of Agriculture, Gifu University
-
MORI Mitsuaki
Nagoya Branch of Japan Food Research Laboratories
-
Sakamoto Eiko
Department Of Agricultural Chemistry Faculty Of Agriculture Gifu University
-
Toukairin-oda Toshikatu
Department Of Agricultural Chemistry Faculty Of Agriculture Gifu University
関連論文
- Effect of Vitamin B_6 Deficiency on the Synthesis and Accumulation of S-Adenosylhomocysteine and S-Adenosylmethionine in Rat Tissues
- Fluorescence Enhancement of PLP for Application to HPLC(Food & Nutrition)
- Effects of Culture Medium on Pyridoxine (Pyridoxamine) Phosphate Oxidase Activity in Wheat Callus(Biological Chemistry)
- Distribution and Properties of Rat Intestinal Alkaline Phosphatase Isoenzymes
- Identification of Rat Serum Alkaline Phosphatase Isoenzyme by measns of Wheat Germ Agglutinin
- Changes of Serum Alkaline Phosphatase Isoenzymes in Fasted Rats
- Comparative Study of Pyridoxine-α, β-Glucosides, and Phosphopyridoxyl-Lysine as a Vitamin B6 Nutrient
- Inactivation of Glucose Oxidase by the Cationic Detergent, Hexadecyltrimethylammonium Bromide
- Distribution of Pyridoxaminephosphate Oxidase Activity in Various Plants and Calluses
- Separation and Characterization of Two Pyridoxamine (Pyridoxine) 5-Phosphate Oxidases from Bakers Yeast
- Induction of Plant Pyridoxine (Pyridoxamine) 5-Phosphate Oxidase Activity by Light
- Essential Histidyl Residues in Glucose Oxidase. Chemical Modification of Histidyl Residues with Diethylpyrocarbonate
- Suppressive Effect of Components in Lemon Juice on Blood Pressure in Spontaneously Hypertensive Rats.
- Separation and Determination of Vitamin B6 Derivatives by Reversed-phase HPLC