Enzymatic Formation of a New Derivative of Thiamin, β-Galactosylthiamin
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概要
- 論文の詳細を見る
A new derivative of thiamin, glycosylated thiamin, was found to be formed from o-nitrophenyl β-D-galactopyranoside and thiamin in 33% CH_3CN by Aspergillus oryzae β-galactosidase. The derivative was isolated in a crystalline form from the reaction mixture by a procedure including PPC, activated vitachange column chromatography, ethanol treatment, PPC, gel-filtration on Toyopearl HW-40S, and lyophilization, and identified as 5'-O-(β-D-galactopyranosyl)-thiamin on the basis of various experimental results, viz. , FAB-MS, UV, IR, ^1H-, and ^<13>C-NMR spectra, thiochrome formation with K_3[Fe(CN)_6]-NaOH reagent, and products by hydrolysis with α- and β-galactosidases. O-β-Galactosylthiamin was odorless and mildly sweet with no stimulative tongue-pricking taste.
- 社団法人日本農芸化学会の論文
- 1994-07-23
著者
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SUZUKI Yukio
Research Institute for Bioresources, Okayama University
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Uchida Kei
Research Institute For Bioresources Okayama University
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Uchida Kei
Research Institute.for Bioresources Okayama University
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Suzuki Yukio
Research Institute.for Bioresources Okayama University
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Suzuki Yukio
Research Insititute For Bioresources Okayama University
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