Phospholipase D-Catalyzed Synthesis of Phosphatidyl Aromatic Compounds
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概要
- 論文の詳細を見る
Phospholipase D (PLD) from Streptomyces sp. was found to catalyze the transfer reaction of the dipalmitoylphosphatidyl residue from 1, 2-dipalmitoyl-3-sn-phosphatidylcholine (DPPC) to an aromatic hydroxy group in a biphasic system of an organic solvent with low water solubility and acetate buffer. The following phosphatidyl aromatic compounds were synthesized : 1, 2-dipalmitoyl-3-sn-phosphatidylphenol(1), 1, 2-dipalmitoyl-3-sn-phosphatidyl-4-methoxyphenol(2, DPP-PMP), 1, 2-dipalmitoyl-3-sn-phosphatidyl-4-methylphenol (3), 1, 2-dipalmitoyl-3-sn-phosphatidylhydroquinone (4), 1, 2-dipalmitoyl-3-sn-phosphatidyl-4-chlorophenol (5), 1, 2-dipalmitoyl-3-sn-phosphatidylcatechol (6), 1, 2-dipalmitoyl-3-sn-phosphatidyl-3-methoxyphenol (7), 1, 2-dipalmitoyl-3-sn-phosphatidyl-2-naphthol (8), and 1, 2-dipalmitoyl-3-sn-phosphatidyl-5-hydroxyindole (9). The transphosphatidylation reaction of DPPC by PLD was accompanied by hydrolysis of DPPC, and the ratios were significantly affected by both organic solvents and water contents in a biphasic system. PLD also catalyzed the transphosphatidylation reaction of DPP-PMP (2) to choline, to reproduce DPPC and liberate 4-methoxyphenol. This is the first report on the enzymatic transfer of phosphatidyl residue to aromatic hydroxy compounds.
- 社団法人日本農芸化学会の論文
- 1994-12-23
著者
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Tsuji M
Hokkaido Univ. Sapporo Jpn
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Suzuki Yoshihito
Dep. Of Applied Biological Chemistry Univ. Of Tokyo
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SUZUKI Yukio
Research Institute for Bioresources, Okayama University
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Suzuki Y
Department Of Global Epidemiology Hokkaido University Research Center For Zoonosis Control
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Suzuki Yoshihito
Department Of Applied Biological Chemistry The University Of Tokyo
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Suzuki Yoshihito
The Institute Of Enology And Viticulture Yamanashi University
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Suzuki Y
Research Institute For Bioresources Okayama University
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HIDAKA Naomi
Research Institute for Bioresources, Okayama University
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TAKAMI Masaaki
Central Research Laboratories, Kuraray Co., Ltd.
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Takami M
Central Research Laboratories Kuraray Co. Ltd.
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Hidaka Naomi
Research Institute For Bioresources Okayama University
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Suzuki Yukio
Research Institute For Bioresources Okayama University
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Suzuki Yukio
Research Insititute For Bioresources Okayama University
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