Enzymatic Preparation of Ginsenosides Rg_2, Rh_1, and F_1

概要

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During investigation of the hydrolysis of a protopanaxatriol-typc saponin mixture by various glycoside hydrolases, crude preparations of β-galactosidase from Aspergillus oryzac and lactase from Penicillium sp. were found to produce two minor saponins, ginsenoside Rg_2 [6-O-(α-1-rhamnopyranosyl-(1→2)-β-D-glucopyranosyl)-20(S)-protopanaxatriol] and ginsenoside Rh_1 (6-O-β-D-glucopyranosyl-20(S)-protopanaxatriol), respectively, in high yields. Moreover, a naringinase preparation from Penicillium decumbens readily gave an intestinal bacterial metabolite, ginsenoside F_1 (20-O-β-D)-glucopyranosyl-20(S)-protopanaxatriol), as the main product, with a small amount of 20(S)-protopanaxatriol from a protopanaxatriol-type saponin mixture. Also, a hesperidinase from Penicillium sp. selectively hydrolyzed ginsenoside Re into ginsenoside Rg_1. This is the first report on the enzymatic preparation of minor saponins, ginsenosides Rg_2, and Rh_1, and of an intestinal bacterial metabolite, ginsenoside F_1, with high efficiency from a protopanaxatriol-type saponin mixture.
公益社団法人日本薬学会の論文
2003-04-01