Further Characterization of Earthworm Serine Proteases : Cleavage Specificity Against Peptide Substrates and on Autolysis
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概要
- 論文の詳細を見る
Cleavage specificity of two fibrinolytic enzymes from Lumbricus rubellus [Nakajima, N., et al., Biosci. Biotechnol. Biochem., 57,1726-1730 (1993) and 60,293-300 (1996)] was investigated using β-amyloid 1-40 and oxidized insulin B-chain as peptide substrates. The serine protease, F-III-2,cleaved the former substrate at six sites, and the latter at five sites. F-II digested them at six and ten, respectively. The cleavage specificity of F-III-2 resembled those of both trypsin and chymotrypsin. F-II had a broader specificity than F-III-2 and preferred also the bonds consisting neutral or hydrophobic amino acids. Furthermore, F-III-2 itself was digested initially on the site of Arg(144)-Tyr(145) to produce two peptide fragments, when it was autolyzed regularly by heating.
- 社団法人日本農芸化学会の論文
- 1999-11-23
著者
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中村 薫
京都大学化学研究所
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中島 伸佳
Graduate School Of Health And Welfare Okayama Prefectural University
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中島 伸佳
岡山県立大学短期大学部
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中島 伸佳
岡山県立短大
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NAKAJIMA Nobuyoshi
Department of Nutritional Science, Okayama Prefectural University
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SUGIMOTO Manabu
Research Institute for Bioresources, Okayama University
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ISHIHARA Kohji
Department of Chemistry, Kyoto University of Education
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NAKAMURA Kaoru
Institute for Chemical Research, Kyoto University
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HAMADA Hiroki
Department of Applied Science, Okayama University of Science
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Hamada H
National Institute Of Fitness And Sports In Kanoya
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中村 薫
京大 化研
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中村 厚三
Univ. Tokyo Tokyo Jpn
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Ishihara K
Dep. Of Life Sci. Okayama Univ. Of Sci. 1-1 Ridai-cho Kita-ku Okayama 700-0005 Jpn
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Nagahama Kazuhiro
Department Of Applied Microbial Technology Faculty Of Biotechnology And Life Science Sojo University
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Nakajima Nobuyoshi
Graduate School Of Health And Welfare Okayama Prefectural University
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Nakajima Nobuyoshi
Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University
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Nagahama Kazuhiro
Department Of Applied Microbial Technology The Kumanoto Institute Of Technology
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Nakamura Kaoru
Institute For Chemical Research Kyoto University
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Sugimoto M
Research Institute For Bioresources Okayama University
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Sugimoto Manabu
Research Institute For Bioresources Okayama University
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Nakamura Kahoru
Department Of Nutrition Tokyo University Of Agriculture
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Hamada Hiroki
Department Of Applied Sci. Okayama University Of Science
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Sugimoto Manabu
Research Instiitte For Bioresources Okayama
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Nakajima Nobuyoshi
Department of Food and Nutrition, Okayama Prefectural Junior College
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HAMADA Hiroki
Department of Applied Sci. , Okayama University of Science
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