Detoxification Effect of Iron-encaging Zeolite-processed Water in Tributyltin-intoxicated Euglena gracilis Z
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概要
- 論文の詳細を見る
In our previous paper, we reported the restoration promoting effects of mineral-encaging zeolite-processed water, especially of a Fe-encaging one, on tributyltin chloride (TBTCl)-intoxicated Euglena gracilis. This present study extends the investigation on the behavior of TBTCl and a xenobiotic enzyme, cytochrome P-450,in Euglena cells incubated with or without Fe-encaging zeolite-processed water (FeZW). Subcellular fractionation of TBTCl-intoxicated Euglena cells, atomic absorption spectrophotometry, and GC analyses showed that TBTCl was rapidly incorporated into the cells to halt cell motility. GC-MS showed that FeZW promoted conversion of TBTCl to dibutyltin (DBT) as the major metabolite in the microsomal fraction of the cells. An in vitro incubation system with heat-treated microsomes did not convert TBTCl to DBT. The contribution of cytochrome P-450 in the microsomal fraction was suggested by an immunochemical method. The results suggest that the improvement of detoxification by FeZW in the TBT-intoxicated Euglena cells should be due to activation of biotransformation system of the Euglena cells by FeZW.
- 社団法人日本農芸化学会の論文
- 2001-01-23
著者
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Suzuki Tomomi
School Of Agriculture Bioscience Center Nagoya University
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NAKAMURA Kaoru
Institute for Chemical Research, Kyoto University
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Sugiyama Tatsuo
Riken Plant Science Center
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Nakamura Kaoru
Institute Of Chemical Research Kyoto University
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OHTA Mari
Division of Marine Life Sciences, Grauate School of Fisheries Science, Hokkaido University
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OHTA Mari
Laboratory of Food Wholesomeness, Department of Marine Bioresources, Faculty of Fisheries, Hokkaido
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KUBO Tomokazu
Laboratory of Food Wholesomeness, Department of Marine Bioresources, Faculty of Fisheries, Hokkaido
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SUZUKI Tetsuya
Laboratory of Food Wholesomeness, Department of Marine Bioresources, Faculty of Fisheries, Hokkaido
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Suzuki T
Division Of Marine Life Sciences Grauate School Of Fisheries Science Hokkaido University
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Nakamura Kaoru
Institute For Chemical Research Kyoto University
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Ohta M
Division Of Marine Life Sciences Grauate School Of Fisheries Science Hokkaido University
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Kubo Tomokazu
Laboratory Of Food Wholesomeness Department Of Marine Bioresources Faculty Of Fisheries Hokkaido Uni
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Suzuki Tetsuya
Laboratory Of Food Hygiene Department Of Food Science And Technology Faculty Of Fisheries Hokkaido U
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Sugiyama Tatsuo
Dept.appl.biol.sci. Sch.agrc.sci. Nagoya Univ.
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