Stereoselective Reduction of Carbonyl Compounds with Actinomycete: Purification and Characterization of Three α-Keto Ester Reductases from Streptomyces avermitilis
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概要
- 論文の詳細を見る
We achieved the purification of three α-keto ester reductases (Streptomyces avermitilis keto ester reductase, SAKERs-I, -II, and -III) from Streptomyces avermitilis NBRC14893 whole cells. The molecular masses of the native SAKERs-I, -II, and -III were estimated to be 72, 38, and 36 kDa, respectively, by gel filtration chromatography. The subunit molecular masses of SAKERs-I, -II, and -III were also estimated to be 32, 32, and 34 kDa, respectively, by SDS-polyacrylamide gel electrophoresis. The purified SAKERs-II and -III showed a reducing activity for α-keto esters (in particular, for ethyl pyruvate). SAKER-I showed a high reducing activity not only toward the α- and β-keto esters, but also toward α-keto acid. The N-terminal region amino acid sequences of SAKERs-I, -II, and -III were identical to that of a putative oxidoreductase, SAV2750, a putative oxidoreductase, SAV1849, and a putative oxidoreductase, SAV4117, respectively, hypothetical proteins coded on the S. avermitilis genome.
- 社団法人 日本農芸化学会の論文
著者
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ISHIHARA Kohji
Department of Chemistry, Kyoto University of Education
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HAMADA Hiroki
Department of Applied Science, Okayama University of Science
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YAMAGUCHI Hitomi
Research and Development Center, Nagase & Co., Ltd.
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NAKAJIMA Nobuyoshi
Graduate School of Health and Welfare Science, Okayama Prefectural University
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Ishihara K
Dep. Of Life Sci. Okayama Univ. Of Sci. 1-1 Ridai-cho Kita-ku Okayama 700-0005 Jpn
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Masuoka Noriyoshi
Department of Life Science, Okayama University of Science
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KATO Chiaki
Graduate School of Science, Okayama University of Science
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IWAI Rieko
Graduate School of Science, Okayama University of Science
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YOSHIDA Momoko
Department of Life Science, Okayama University of Science
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IKEDA Natsumi
Department of Life Science, Okayama University of Science
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