Purification and Characterization of Konjac Glucomannan Degrading Enzyme from Anaerobic Human Intestinal Bacterium, Clostridium butyricum-Clostridium beijerinckii Group
スポンサーリンク
概要
- 論文の詳細を見る
Konjac glucomannan degrading enzyme was purified to homogeneity from the culture broth of an anaerobic human intestinal bacterium, Clostridium butyricum-Clostridium beijerinckii group. The enzyme was composed of a single polypeptide chain with a molecular weight of 50,000-53,000. The enzyme was an endo-β-mannanase that acted specifically on the polysaccharides such as konjac glucomannan and coffee mannan, producing exclusively their smaller oligosaccharides and the monosaccharides. The optimal pH of the enzyme for the hydrolysis of konjac glucomannan was around 7-8 and the enzyme was stable in rather alkaline pH range of 8-10. The enzyme reaction was activated by the addition of CaCl_2 and dithiothreitol. It was suggested that the enzyme might contribute to the decomposition of konjac glucomannan in human digestive tract.
- 1997-10-23
著者
-
NAKAJIMA Nobuyoshi
Department of Nutritional Science, Okayama Prefectural University
-
MATSUURA Yasushi
Department of Materials Science and Chemistry, Himeji Institute of Technology, University of Hyogo
-
Nakajima Nobuyoshi
Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University
-
Matsuura Yasushi
Department Of Food Science Okayama Prefectural Junior College
-
Matsuura Y
Okayama Prefectural Univ. Okayama Jpn
-
Nakajima Nobuyoshi
Department of Food and Nutrition, Okayama Prefectural Junior College
関連論文
- Further Characterization of Earthworm Serine Proteases : Cleavage Specificity Against Peptide Substrates and on Autolysis
- Multi-enzymatic Glucosylation Using Eucalyptus UDP-Glucosyltransferase Coupled UDPglucose-Fermentation by Bakers'Yeast
- Lipase-catalyzed Synthesis of Arbutin Cinnamate in an Organic Solvent and Application of Transesterification to Stabilize Plant Pigments
- Preparation of Optically Active α-Hydroxy Esters : Stereoselective Reduction of α-Keto Esters Using Thermophilic Actinomycetes
- Amino Acid Sequence and Characterization of Aldo-keto Reductase from Bakers' Yeast
- Stereoselective Reduction of α- and β-Keto Esters with Aerobic Thermophiles, Bacillus Strains
- Protein Sequences of Two Keto Ester Reductases: Possible Identity as Hypothetical Proteins
- Enzymatic Production of (S)-1-Acetoxy-2-Alkanol with Bakers' Yeast Cell-Free Extract in a Membrane Reactor
- Multi-enzymatic Glucosylation Using Eucalyptus UDP-Glucosyltransferase Coupled UDPglucose-Fermentation by Bakers'Yeast
- Amino Acid Sequence and Characterization of Aldo-keto Reductase from Bakers' Yeast
- Enzymatic Production of (S)-1-Acetoxy-2-Alkanol with Bakers' Yeast Cell-Free Extract in a Membrane Reactor
- A Novel Zinc-containing α-Keto Ester Reductase from Actinomycete : An Approach Based on Protein Chemistry and Bioinformatics
- Control of Thermally Enhanced Photoinduced Reorientation Direction of Photocrosslinkable Copolymer Liquid Crystals and Application to Polarization Gratings Using Linearly Polarized Ultraviolet Light
- A fibrinolytic enzyme from the green alga Codium latum activates plasminogen
- Degradation of Pectic Substances by Two Pectate Lyases from a Human Intestinal Bacterium, Clostridium butyricum-beijerinckii Group
- Modified Alcohol Oxidase Method Used to Determine the Degree of Pectin Methylesterification
- Purification and Characterization of α-Keto Ester Reductases from Bakers' Yeast
- Differences in Protein Structure and Similarities in Catalytic Function of Two L-Stereoselective Carbonyl Reductases from Bakers' Yeast
- Enzymatic preparation of arbutin derivatives : Lipase-catalyzed direct acylation without the need of vinyl ester as an acyl donor(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Synthesis of Unnatural Mono- and Oligosaccharides of Farnesol, Geraniol, and (S)-Perillyl Alcohol by Biocatalytic Glycosylations
- Enhancement of Water-Solubility and Bioactivity of Paclitaxel Using Modified Cyclodextrins(METHODS)
- 有機溶媒に耐性で常温においては自己消化による失活を伴わない「ミミズプロテアーゼ」の物質合成・変換への応用 (特集 物質合成の設計図にブレークスルーを--酵素触媒の活躍と未来)
- Analysis of Isoflavone Content in Tempeh, a Fermented Soybean, and Preparation of a New Isoflavone-Enriched Tempeh(BREWING AND FOOD TECHNOLOGY)
- An Isozyme of Earthworm Serine Proteases Acts on Hydrolysis of Triacylglycerol
- Biotransformation of (+)-catechin by plant cultured cells of Eucalyptus perriniana
- Purification and Characterization of α-Keto Amide Reductase from Saccharomyces cerevisiae
- Stereoselective biotransformation of limonene and limonene oxide by cyanobacterium, Synechococcus sp. PCC 7942
- Damage to Cultivated Japanese Pearl Oysters by Oxidative Stress That Was Related to "Mass Mortality"(Biochemistry & Molecular Biology)
- One- step Glucosylation of Capsaicinoids by Cultured Cells of Phytolacca americana
- Comparison of Acylated Plant Pigments : Light-resistance and Radical-scavenging Ability(Biochemistry & Molecular Biology)
- Further Stabilization of Earthworm Serine Protease by Chemical Modification and Immobilization(Biochemistry & Molecular Biology)
- Enzyme-catalyzed Acylation of Plant Polyphenols for Interpretation of Their Functions
- Stereoselective Reduction of Ketone and Enone using Plant Cell Cultures
- Purification and Characterization of Two α-Keto Ester Reductases from Streptomyces thermocyaneoviolaceus IFO 14271(Microbiology & Fermentation Technology)
- Functional Glucosylation of Kojic Acid and Daidzein with the Eucalyptus Membrane-Associated UDP-Glucosyltransferase Reaction System
- Stereocontrolled Reduction of α-and β-Keto Esters with Micro Green Algae, Chlorella Strains
- Regioselective Acylation of Flavonoid Glucoside with Aromatic Acid by an Enzymatic Reaction System from Cultured Cells of Ipomoea batatas
- Purification and Characterization of Konjac Glucomannan Degrading Enzyme from Anaerobic Human Intestinal Bacterium, Clostridium butyricum-Clostridium beijerinckii Group
- Chemical Modification of Earthworm Fibrinolytic Enzyme with Human Serum Albumin Fragment and Characterization of the Protease as a Therapeutic Enzyme
- Stable Earthworm Serine Proteases : Application of the Protease Function and Usefulness of the Earthworm Autolysate
- Weakly Acidic Pectic Polysaccharides of Japanese Radish and Cabbage(Biological Chemistry)
- Isolation and Characterization of a Xylose-rich Pectic Polysaccharide from Japanese Radish(Biological Chemistry)
- Purification and Characterization of Aldehyde Reductase from Leuconostoc dextranicum
- Pectic Acid Degrading Enzymes from Human Feces(Biological Chemistry)
- Matrix Polysaccharides Involved in Adhesion of the Cell Walls of Kidney Bean Cotyledon Tissues(Biological Chemistry)
- Biotransformation of Cinnamic Acid, p-Coumaric Acid, Caffeic Acid, and Ferulic Acid by Plant Cell Cultures of Eucalyptus perriniana
- Stereoselective Reduction of Carbonyl Compounds with Actinomycete : Purification and Characterization of Three α-Keto Ester Reductases from Streptomyces avermitilis
- Characterization of Potent Fibrinolytic Enzymes in Earthworm, Lumbricus rubellus
- Enzymatic Production of β-Thujaplicin 2-O-β-D-Glucoside in a Membrane Reactor Using an Insoluble UDP-Glucosyltransferase Fraction from Cultured Cells of Eucalyptus perriniana
- Lipase-Catalyzed Direct and Regioselective Acylation of Flavonoid Glucoside for Mechanistic Investigation of Stable Plant Pigments
- Potent Fibrinolytic Enzyme from the Lysate of Katsuwonus pelamis Digestive Tract (Shiokara) : Purification and Characterization
- Degradation of Konjac Glucomannan by Enzymes in Human Feces and Formation of Short-Chain Fatty Acids by Intestinal Anaerobic Bacteria
- Control of Thermally Enhanced Photoinduced Reorientation Direction of Photocrosslinkable Copolymer Liquid Crystals and Application to Polarization Gratings Using Linearly Polarized Ultraviolet Light
- Experimental Studies upon the Reciprocal Rhythm
- Stereochemical Control in Microbial Reduction. 18. Mechanism of Stereochemical Control in the Diastereoselective Reduction with Bakers' Yeast.