Characterization of Potent Fibrinolytic Enzymes in Earthworm, Lumbricus rubellus
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概要
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Stable and potent fibrinolytic enzymes (six homogeneous proteins) were purified to homogeneity from extracts of the lyophilized powder of an earthworm, Lumbricus rubellus. The molecular weight of each enzyme estimated by SDS-polyacrylamide gel electrophoresis was different from those by gel filtration chromatography in the six purified proteins. The exact molecular weight of each enzyme (F-III-2, F-III-1, F-II, F-I-2, F-I-1, and F-I-0) measured by ionspray MS analysis was 29,662, 29,667, 24,664, 24,220, 24,196, and 23,013, respectively. The isoelectric point (pI) of each enzyme was 3.40, 3.60, 4.20, 4.00, 4.30, and 4.85, respectively. The enzymes were single polypeptide chains. They had a very strong fibrinolytic activity and the maximum reactivity for chromogenic substrates from pH 9-11. The enzymes, acidic proteins that had abundant asparagine and aspartic acid, and low lysine in their amino acid composition, did not contain component sugars. The enzymes were stable at from pH 1-11 and up to 60℃. Studies on substrate specificity and inhibition indicated that these enzymes were alkaline trypsin-like serine proteases. N-Terminal amino acid sequences of the enzymes had local similarities to those of trypsin-like enzymes such as elastase and coagulation factor IX. From the results of amino acid sequence, amino acid composition analyses and immunological analyses, it was suggested that these six enzyme proteins were derived as isozyme(s) from at least four different genes.
- 社団法人日本農芸化学会の論文
- 1993-10-23
著者
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Nakajima Nobuyoshi
Department Of Nutritional Science Faculty Of Health And Welfare Okayama Prefectural University
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Sumi Hiroyuki
Department Of Physiological Chemistry Kurashiki University Of Science And The Arts
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Mihara Hisashi
Department Of Physiology Miyazaki Medical College
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Sumi Hiroyuki
Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Univ.
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Nakajima Nobuyoshi
Department Of Nutritional Science Faculty Of Health And Welfare Science Okayama Prefectural Univ.
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Nakajima Nobuyoshi
Department of Food and Nutrition, Okayama Prefectural Junior College
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