A novel hydroxylase from Catharanthus roseus participating in the hydroxylation of 2-hydroxybenzoic acid

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概要

• 論文の詳細を見る

• A novel 55-kDa hydroxylase was isolated from cultured cells of Catharanthus roseus by a three-step procedure: anion exchange chromatography, affinity chromatography and hydroxylapatite adsorption chromatography. The enzyme specifically catalyzed the hydroxylation of 2-hydroxybenzoic acid to give 2,5-dihydroxybenzoic acid. The enzyme activity was optimal at pH 7.8 and was completely inhibited by divalent cations, such as Cu2+ and Hg 2+. The enzyme showed sequence similarity to certain plant flavonoid 3′-hydroxylases.

• 社団法人日本生物工学会の論文
• 2004-08-25

著者

• Hamada H

  National Institute Of Fitness And Sports In Kanoya

• Shimoda Kei

  Department of Chemistry, Faculty of Medicine, Oita University

• Kubota Naoji

  Department of Chemistry, Faculty of Medicine, Oita University

• Hirota Tomoko

  Department Of Mathematical And Life Science Graduate School Of Sciense Hiroshima University

• HIRATA Toshifumi

  Department of Chemistry, Faculty of Science, Hiroshima University

• Hamada Hiroki

  Materials And Devices Development Center Bu Sanyo Electric Co. Ltd.

• SANO TAKAYUKI

  Department of Pharmacology and Therapeutics, Faculty of Medicine, Oita University

• HIRAKAWA HIDETADA

  Department of Cell Membrane Biology, Institute of Scientific and Industrial Research, Faculty of Pha

• Sano Tetsuya

  Department Of Fine Materials Engineering Faculty Of Textile Science And Technology Shinshu Universit

• Shimoda K

  Hiroshima Univ. Higashi‐hiroshima

• Shimoda Kei

  Department Of Chemistry Faculty Of Medicine Oita University

• Kubota Naoji

  Department Of Applied Chemistry Faculty Of Engineering Oita University

• Hirata T

  Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University

• Hirata T

  Hiroshima Univ. Higashi‐hiroshima Jpn

• Hirata Toshifumi

  Dep. Of Mathematical And Life Sciences Graduate School Of Sci. Hiroshima Univ.

• Sano Takayuki

  Department Of Pharmacology And Therapeutics Faculty Of Medicine Oita University

• Kubota N

  Department Of Chemistry Faculty Of Medicine Oita University

• Sano Takayuki

  Department Of Materials Science Faculty Of Science Hiroshima University

• Shimoda K

  Department Of Chemistry Faculty Of Medicine Oita University

• Hirakawa Hidehiko

  Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo

• Hirata Toshifumi

  Department Of Chemistry Faculty Of Science Hiroshima University

• Sano Takayuki

  Department of Chemistry, Faculty of Science, Hiroshima University

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