Properties of an Alcohol Dehydrogenase from the Hyperthermophilic Archaeon Aeropyrum pernix K1 (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)

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概要

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• A NAD^+-dependent medium-chain alcohol dehydrogenase from the hyperthermophilic archaeon Aeropyrum pernix K1 was expressed in Escherichia coli and purified. The recombinant enzyme was a homotetramer of molecular mass 1.6×10^2 kDa. The optimum pH for the oxidative reaction was around 10.5 and that for the reductive reaction was around 8.0. The enzyme had a broad substrate specificity including aliphatic and aromatic alcohols, aliphatic and aromatic ketones, and benzylaldehyde. This enzyme produced (S)-alcohols from the corresponding ketones. The enzyme was thermophilic and the catalytic activity increased up to 95℃. It maintained 24% of the original catalytic activity after incubation for 30 min at 98℃, indicating that this enzyme is highly thermostable.

• 社団法人日本生物工学会の論文
• 2004-03-25

著者

• Nagamune Teruyuki

  Department of Bioengineering, Graduate School of Engineering, The University of Tokyo

• Nagamune T

  Univ. Tokyo Tokyo Jpn

• Nagamune T

  Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo

• Nagamune Teruyuki

  Dep. Of Chemistry And Biotechnology Graduate School Of Engineering The Univ. Of Tokyo 7-3-1 Hongo Bu

• Nagamune Teruyuki

  Department Of Bioengineering Graduate School Of Engineering The University Of Tokyo:department Of Ch

• Nagamune Teruyuki

  Department Of Bioengineering Center For Nanobio Integration The University Of Tokyo:department Of Ch

• Kamiya Noriho

  Department of Applied Chemistry, Graduate School of Engineering, Kyushu University

• Kamiya Noriho

  Department Of Applied Chemistry Graduate School Of Engineering Kyushu University

• Kamiya Noriho

  Department Of Applied Chemistry Faculty Of Engineering Kyushu University

• HIRAKAWA HIDEHIKO

  Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo

• KAWARABAYASHI YUTAKA

  Research Center for Glycoscience National Institute of Advancd Industrial Science and Technology

• Nagamune Teruyuki

  Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo:dep

• Hirakawa Hidehiko

  Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo

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