Synergistic effects of detergents and organic solvents on protein refolding: Control of aggregation and folding rates(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
This paper presents the synergistic enhancement of the refolding yield of denatured and reduced lysozyme by using detergents as aggregation inhibitors and water-miscible organic cosolvents as modulators for the detergents. Adding only cetyltrimethylammonium bromide (CTAB) led to a slight increase in the refolding yield (up to 13%). Further addition of dimethylsulfoxide (DMSO) with CTAB drastically increased the refolding yield up to 35%, a value which was higher than the simple sum of the refolding yields in the presence of only CTAB or DMSO. The synergistic enhancement was also observed in the coexistence of other detergents, such as polyethylene glycol monooleyl ether (n=50) and N-tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate, and cosolvents, such as N,N-dimethylformamide and N,N-dimethylacetamide. Experimental data and a kinetic analysis revealed the guideline for selecting a couple of additives; detergents which can adequately inhibit the aggregation of proteins by binding to hydrophobic surfaces of refolding intermediates should be employed as an aggregation inhibitor, and cosolvents which can properly prevent both protein-protein and protein-detergent interactions act as effective modulators for the aggregation inhibitor, resulting in a desirable balance between folding and aggregation rates.
- 社団法人日本生物工学会の論文
著者
-
Nagamune Teruyuki
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo
-
YAMAGUCHI SATOSHI
Department of Surgery, Nagasaki Prefectural Shimabara Hospital
-
Nagamune Teruyuki
Dep. Of Bioengineering School Of Engineering Univ. Of Tokyo Tokyo 113-8656 Jpn
-
Nagamune Teruyuki
Dep. Of Bioengineering Graduate School Of Engineering The Univ. Of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo
-
Nagamune Teruyuki
Department Of Bioengineering Graduate School Of Engineering The University Of Tokyo:department Of Ch
-
Nagamune Teruyuki
Department Of Bioengineering Center For Nanobio Integration The University Of Tokyo:department Of Ch
-
Nagamune Teruyuki
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo:dep
-
Yamamoto Etsushi
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
-
Yamamoto Etsushi
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
-
Yamaguchi Satoshi
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
-
Yamaguchi Satoshi
Department Of Applied Chemistry Graduate School Of Engineering Kyushu University
-
Yamaguchi Satoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
関連論文
- Comparison of efficiency of terminal differentiation of oligodendrocytes from induced pluripotent stem cells versus embryonic stem cells in vitro(CELL AND TISSUE ENGINEERING)
- BM-P3 Fluorescein-responsive migration of mammalian cells equipped with an antibody/receptor chimera(Section III Biomedical Engineering)
- An Unusual Perforation of the Colon : Report of Two Cases
- Impaction of a Shrapnel Splinter in the Common Bile Duct After Migrating from the Right Thoracic Cavity : Report of a Case
- Successful Repeated Transcatheter Arterial Embolization (TAE) for Multiple Liver Metastases from Breast Cancer
- Development of an Off-axis Sample Introduction System for Use in Multiphoton Ionization/Time-of-flight Mass Spectrometry
- Gas Chromatography/Multiphoton Ionization/Mass Spectrometry of Polychlorinated Dibenzofurans Using Nanosecond and Femtosecond Lasers
- Development of a multi-layered virtual tooth model for the haptic dental training system
- A Low Invasive Gene Transfer to a Single Cell Using Nanoneedle Operated by AFM
- 2P564 A low invasive and high efficient gene delivery method into a single cell using nanoneedle and AFM(53. Bioengineering,Poster Session,Abstract,Meeting Program of EABS & BSJ 2006)
- MN-O1 A caged phosphopeptide-based approach for photochemical activation of kinases in living cells(Section X Micro/Nano Technology for Analysis and Cell Manipulation)
- Monolayer Formation of Cyotchrome b_ on Gold Surfaces and Its Reconstitution Reaction, Studied by Surfaces Plasmon Resonance
- Monomolecular Layer of the Alkylated Cytochrome b_ Formed at the Air-Water Interface
- Bone regeneration using collagen type I vitrigel with bone morphogenetic protein-2(MEDICAL BIOTECHNOLOGY)
- Ruptured Aneurysm of the Visceral Artery : Report of Two Cases
- Ligamentum Flavum Hematoma in the Lumbar Spine : Case Report
- Multiple Vertebral Metastases from Malignant Cardiac Pheochromocytoma
- Case of clear cell ependymoma of medulla oblongata : Clinicopathological and immunohistochemical study with literature review
- Eradication and reinfection of human papillomavirus after photodynamic therapy for cervical intraepithelial neoplasia
- Ovarian malignant mixed mesodermal tumor producing the free form of the β-subunit of human chorionic gonadotropin
- Induction of apoptosis and manganese superoxide dismutase gene by photodynamic therapy in cervical carcinoma cell lines
- Intradiploic Arachnoid Cyst Identified by Diffusion- Weighted Magnetic Resonance Imaging
- Exogenous gene expression and growth regulation of hematopoietic cells via a novel human artificial chromosome
- Gastric Necrosis After an Infarction of the Spleen : Report of a Case
- Attenuated Familial Adenomatous Polyposis Associated with Advanced Rectal Cancer in a 16-Year-Old Boy : Report of a Case
- Small-Cell Carcinoma of the Gallbladder : Report of a Case
- Intrahepatic Cholangiocarcinoma with Extensive Sarcomatous Change: Report of a Case
- Spinal Epidural Granulocytic Sarcoma in a Child Precedent to Clinical Manifestation of Acute Myeloid Lymphoma : Case Report
- Spinal Surgery in Nepal : A Brief Introduction
- Comparative study on novel endoluminal ultrasonography and conventional transurethral ultrasonography in staging of bladder cancer
- Penetrating Injury of the Upper Cervical Spine by a Chopstick : Case Report
- Simultaneous Cranial and Spinal Subdural Hematoma : Case Report
- Microbial Ecology of Nitrifying Bacteria in Wastewater Treatment Process Examined by Fluorescence In Sity Hybridization
- Ribosomal RNA Supplementation Highly Reinforced Cell-Free Translation Activity of Wheat Germ
- Construction, Bacterial Expression, and Characterization of Hapten-Specific Single-Chain Fv and Alkaline Phosphatase Fusion Protein
- Gene Organization and Low Regiospecificity in Aromatic-Ring Hydroxylation of a Benzene Monooxygenase of Pseudomonas aeruginosa JI104
- Truncation of Vargula Lucifersae Still Results in Retention of Luminescence
- Enhancement of Mutation Frequency with Nucleotide Triphosphate Analogs in PCR Random Mutagenesis
- Comparison of 4MV photon surface dose among Varian, Siemens, and Elekta linear accelerators for tangential breast treatment : a phantom study
- Synthesis of HIV Protease Dipeptide Inhibitors and Prodrugs
- Cloning and Characterization of Extradiol Aromatic Ring-Cleavage Dioxygenases of Pseudomonas aeruginosa JI104
- Effect of Culture Temperature Shift on the Cellular Sugar Accumulation of Chlorella vulgaris SO-26
- AN-P34 The generation of oligodendrocytes from induced pluripotent stem cells(Section I Animal and Plant Cell Cultures)
- A New Method of ATP Regeneration Rate Measurement Using a pH Meter
- Video-Assisted Thoracoscopic Diverticulectomy of the Midesophagus
- Mimicry of Erythropoietin and Interleukin-6 Signalling by an Antibody/Cytokine Receptor Chimera in Murine Myeloid 32D Cells
- Antigen-Mediated Genetically Modified Cell Amplification (AMEGA) with Single Vector Transduction
- Selection of Highly Productive Mammalian Cells Based on an Inducible Growth Advantage Using an Antibody/Receptor Chimera
- A Growth Signal with an Artificially Induced Erythropoietin Receptor-gp130 Cytoplasmic Domain Heterodimer
- Importance of a CDR H3 Basal Residue in V_H/V_L Interaction of Human Antibodies
- Properties of an Alcohol Dehydrogenase from the Hyperthermophilic Archaeon Aeropyrum pernix K1 (ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Cryptococcus arboriformis Sp. Nov., a Novel Anamorphic Basidiomycetous Yeast Species Isolated from a Patient's Urine
- The Influence of Domain Structures on the Signal Transduction of Chimeric Receptors Derived from the Erythropoietin Receptor
- Detection of Protein-Protein Interaction by Bioluminescence Resonance Energy Transfer from Firefly Luciferase to Red Fluorescent Protein
- Rapid Homogeneous Immunoassay of Peptides Based on Bioluminescence Resonance Energy Transfer from Firefly Luciferase
- Genetically Engineered Active Qβ Replicase in Rabbit Reticulocyte Cell-Free System : a Fusion Protein of EF-Tu and EF-Ts Is Functional as the Subunit of Qβ Replicase
- Single Cell Reporter Assay Using Cell Surface Displayed Vargula Luciferase
- Expanded-Bed Protein Refolding Using a Solid-Phase Artificial Chaperone
- Monitoring of the Refolding Process for Immobilized Firefly Luciferase with a Biosensor Based on Surface Plasmon Resonance
- Refoldin of Firefly Luciferase Immobilized on Agarose Beads
- Construction of Chimeric Proteins between Protein G and Fluorescence-Enhanced Green Fluorescent Protein, and Their Application to Immunoassays
- Synergistic effects of detergents and organic solvents on protein refolding: Control of aggregation and folding rates(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- EP-P33 Development of small molecular additives for protein refolding and proteomics technology(Section VII Enzyme and Protein Engineering)
- Cell Adhesion Ability of Artificial Extracellular Matrix Proteins Containing a Long Repetitive Arg-Gly-Asp Sequence(CELL AND TISSUE ENGINEERING, Advances in Biomedical Science and Engineering)
- Novel Light-Illumination Scanning Tunneling Microscopy Equipped with Optical Fiber Probe
- Study of a rat model for calcium oxalate crystal formation without severe renal damage in selected conditions
- Calcium oxalate monohydrate crystal binding substance produced from Madin-Darby canine kidney cells
- Construction of Green Fluorescent Protein Reporter Genes for Genotoxicity Test(SOS/umu-Test)and Improvement of Mutagen-Sensitivity
- OJ-059 Plasma Heparin Cofactor II Activity Predicts Cardio-and Cerebrovascular Events in Patients with Coronary Heart Disease(Preventive medicine/Epidemiology/Education-2 (H) OJ10,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting
- Conducting Polymer-coated Electrode as a Reference/Counter Electrode in an Organic Phase and Its Application to a Two-electrode Type Thin-layer Cell for Voltammetry at the Liquid | Liquid Interface
- Fournier gangrene associated with hyper IgE syndrome (Job syndrome)
- Improvement of stone comminution by slow delivery rate of shock waves in extracorporeal lithotripsy
- Electron Transfer from Zn-Protoporphyrin IX to Ruthenium Ammine Attached at His63 of Reconstituted Cytochrome b_
- Development of a multi-layered virtual tooth model for the haptic dental training system
- Solitary Fibrous Tumors of the Pleura: Report of Two Cases Resected by Video-assisted Thoracoscopic Surgery
- Fabrication of Protein Microarrays for Immunoassay Using the Electrospray Deposition (ESD) Method
- Development of a homogeneous competitive immunoassay for phosphorylated protein antigen based on the enhanced fluorescence resonance energy transfer technology(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Lateral Buttock Congenital Dermal Sinus Tract : Case Report
- Utility of Direct Stimulation of Roots in Spinal Surgery
- Synthesis and Insecticidal Activity of Lignan Analogs (1)(Pesticide Chemistry)
- Application of the Random Amplified Polymorphic DNA(RAPD)Technique to Distinguishing Species of the Red Tide Phytoplankton Chattonella(Raphydophyceae)
- BP-P7 Construction of rapid antibody isolation system and development of the novel biomarker using RAntIS(Section II Biopharmaceuticals Production)
- Identification of bikunin isolated from human urine inhibits calcium oxalate crystal growth and its localization in the kidneys
- SOLVENT EXTRACTION OF INDIUM WITH TRIALKYLPHOSPHINE OXIDE FROM SULFURIC ACID SOLUTIONS CONTAINING CHLORIDE ION
- Comparison of MRI-based assessment systems for osteoarthritic knees : the irregularity index system and WORMS
- A New Nonlinear Integrator with Positive Phase Shifts
- Editorial comment
- Growth control of genetically modified cells using an antibody/c-Kit chimera(CELL AND TISSUE ENGINEERING)
- Vertically Y-Branched Mode Splitter/Combiner for Intraboard Chip-to-Chip Optical Interconnection with Wavelength-Division Multiplexing
- Novel Light-Illumination Scanning Tunneling Microscopy Equipped with Optical Fiber Probe
- Growth control of genetically modified cells using an antibody/c-Kit chimera
- Development of three-dimensional patient face model that enables real-time collision detection and cutting operation for a dental simulator
- Analysis of cellular phenotype during in vitro immunization of murine splenocytes for generating antigen-specific immunoglobulin(METHODS)
- Molecular recognition moiety and its target biomolecule interact in switching enzyme activity(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Surgical Improvement of Speech Disorder Caused by Amyotrophic Lateral Sclerosis
- Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Effect of length of molecular recognition moiety on enzymatic activity switching(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Molecular recognition moiety and its target biomolecule interact in switching enzyme activity
- Zero-dark-counting high-speed X-ray photon detection using a cerium-doped yttrium aluminum perovskite crystal and a small photomultiplier tube and its application to gadolinium imaging