Construction of Green Fluorescent Protein Reporter Genes for Genotoxicity Test(SOS/umu-Test)and Improvement of Mutagen-Sensitivity
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概要
- 論文の詳細を見る
Green fluorescent protein(GFP)reporter genes for the bacterial umu-test were constructed. Utilization of tandem, lacUV5 and chimeric trp/umu promoters, and coexpression of the Escherichia coli recA5327 mutant enhanced the GFP expression level fourteen-fold over that of the system with only the umu promoter, thereby improving the sensitivity of the umu-test.
- 社団法人日本生物工学会の論文
- 2001-09-25
著者
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Oda Yoshimitsu
Osaka Prefectural Institute of Public Health
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Nagamune Teruyuki
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo
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ARAI Ryoichi
Department of Physics, Saitama University
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Arai R
Department Of Zoology University Museum University Of Tokyo
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Arai Ryoichi
Dep. Of Zoology Univ. Museum Univ. Of Tokyo
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Arai Ryoichi
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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Nagamune Teruyuki
Department Of Bioengineering Center For Nanobio Integration The University Of Tokyo:department Of Ch
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Arai Ryoichi
Intl. Young Res. Empow. Ctr. Shinshu Univ.:riken Ssbc
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Nagamune Teruyuki
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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MAKITA YUKIMASA
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
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Arai Ryoichi
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Makita Yukimasa
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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