Rapid Homogeneous Immunoassay of Peptides Based on Bioluminescence Resonance Energy Transfer from Firefly Luciferase

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概要

• 論文の詳細を見る

• A sensitive homogeneous immunoassay is needed in the field of clinical diagnostics. Here we propose a rapid and potentially sensitive homogeneous immunoassay of peptide epitope based on the bioluminescence resonance energy transfer (BRET). A GST-peptide tag fused to the N-terminus of firefly luciferase, and Cy3 or Cy3.5-labeled anti-peptide antibody were prepared to detect BRET from the hybrid luciferase to the fluorolabeled antibody. By measuring the spectral change due to BRET, the amount of c-myc peptide was successfully determined in a short period.

• 社団法人日本生物工学会の論文
• 2002-06-20

著者

• UEDA Hiroshi

  Department of Pathology, School of Medicine Gifu University

• Nagamune Teruyuki

  Department of Bioengineering, Graduate School of Engineering, The University of Tokyo

• Nagamune T

  Univ. Tokyo Tokyo Jpn

• Nagamune T

  Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo

• Nagamune Teruyuki

  Dep. Of Chemistry And Biotechnology Graduate School Of Engineering The Univ. Of Tokyo 7-3-1 Hongo Bu

• Nagamune Teruyuki

  Department Of Bioengineering Graduate School Of Engineering The University Of Tokyo:department Of Ch

• Nagamune Teruyuki

  Department Of Bioengineering Center For Nanobio Integration The University Of Tokyo:department Of Ch

• KITAYAMA ATSUSHI

  Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo

• 上田 宏

  東京大学大学院工学研究科

• Ueda Hiroshi

  Department Of Dermatology Fujita Health University School Of Medicine

• Nagamune Teruyuki

  Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo:dep

• YAMAKAWA YUKARI

  Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo

• Kitayama A

  Department Of Chemistry And Biotechnology Graduate School Of Engineering University Of Tokyo:(presen

• Kitayama Atsushi

  Department Of Chemistry And Biotechnology The University Of Tokyo

• Yamakawa Yukari

  Department Of Chemistry And Biotechnology Graduate School Of Engineering University Of Tokyo

• Ueda Hiroshi

  Department Of Anatomy University Of Occupational And Environmental Health School Of Medicine

• Ueda Hiroshi

  Department Of Anatomy School Of Medicine University Of Occupational And Environmental Health

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