Overexpression of bcl-2 Improved Survival of Cos-1 Cells and Enhanced Transient Protein Production

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概要

• 論文の詳細を見る

• COS-1 cells were transfected with human bcl-2 gene by using the plasmid vector BCMGS-neo derived from bovine papilloma virus type-1; the plasmid can highly replicate in the nucleus of mammalian cells. The transfectants overexpressed human Bcl-2 and showed remarkably improved survival when cultured at low (0.2%) serum concentration, where apoptosis normally occurs. For examining the efficiency of protein production, the bcl-2 transfectants were further transfected with mouse lambda chain gene carried by an SV40-derived plasmid for transient expression. The control cells stopped production on day 6,but bcl-2 transfectants continued producing longer and a twofold amount of the lambda protein was obtained, presumably because Bcl-2 suppressed apoptosis in late culture. These COS-1 cells stably overexpressing Bcl-2 will be advantageous for transient expression of various recombinant proteins.

• 社団法人日本生物工学会の論文
• 1996-12-25

著者

• UEDA Hiroshi

  Department of Pathology, School of Medicine Gifu University

• Suzuki Eiji

  Department of Internal Medicine II, Fukushima Medical University School of Medicine, Fukushima

• Terada Satoshi

  Department of Applied Chemistry and Biotechnology, University of Fukui

• Fujita Tetsuo

  Department Of Physics Nagoya University

• Suzuki E

  Shinshu Univ. Ueda Jpn

• Terada Satoshi

  Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo

• Terada Satoshi

  Department Of Applied Chemistry And Biotechnology Faculty Of Engineering University Of Fukui

• Fujita T

  Institute Of Applied Biochemistry University Of Tsukuba

• SUZUKI Eiji

  TDK Corporation

• Fujita Tetsuo

  Department Of Electronic Engineering Shizuoka Institute Of Science And Technology

• Suzuki Eiji

  Department Of Fine Materials Engineering Faculty Of Textile Science & Technology Shinshu Univers

• Terada Shoichi

  Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo

• Ueda Hiroshi

  Department Of Dermatology Fujita Health University School Of Medicine

• Suzuki E

  Tdk Corporation

• Suzuki Eiji

  Department Of Biochemistry Faculy Of Veterinary Medicine Hokkaido University

• Ueda Hiroshi

  Department Of Anatomy University Of Occupational And Environmental Health School Of Medicine

• Ueda Hiroshi

  Department Of Chemistry And Biotechnology Faculty Of Engineering University Of Tokyo

• Ueda Hiroshi

  Department Of Anatomy School Of Medicine University Of Occupational And Environmental Health

• Fujita Tetsuo

  Department of Applied Chemistry, Faculty of Engineering, Osaka University

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