Detection of Protein-Protein Interaction by Bioluminescence Resonance Energy Transfer from Firefly Luciferase to Red Fluorescent Protein
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概要
- 論文の詳細を見る
We describe a novel homogeneous protein-protein interaction assay based on bioluminescence resonance energy transfer (BRET) from mutant firefly luciferase to red fluorescent protein (DsRed). The association of glutathione S-transferase (GST)-luciferase and protein G-DsRed, which was driven by the addition of anti-GST antibody, was successfully monitored by BRET.
- 社団法人日本生物工学会の論文
- 2002-10-25
著者
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UEDA Hiroshi
Department of Pathology, School of Medicine Gifu University
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Nagamune Teruyuki
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo
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ARAI Ryoichi
Department of Physics, Saitama University
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Arai R
Department Of Zoology University Museum University Of Tokyo
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Arai Ryoichi
Dep. Of Zoology Univ. Museum Univ. Of Tokyo
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Arai Ryoichi
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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NAKAGAWA Hideyuki
Department of Electrical and Electronics Engineering, Fukui University
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Nagamune T
Univ. Tokyo Tokyo Jpn
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Nagamune T
Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo
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Nagamune Teruyuki
Dep. Of Chemistry And Biotechnology Graduate School Of Engineering The Univ. Of Tokyo 7-3-1 Hongo Bu
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Nagamune Teruyuki
Department Of Bioengineering Graduate School Of Engineering The University Of Tokyo:department Of Ch
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Nagamune Teruyuki
Department Of Bioengineering Center For Nanobio Integration The University Of Tokyo:department Of Ch
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Arai Ryoichi
Intl. Young Res. Empow. Ctr. Shinshu Univ.:riken Ssbc
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KITAYAMA ATSUSHI
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
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上田 宏
東京大学大学院工学研究科
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Ueda Hiroshi
Department Of Dermatology Fujita Health University School Of Medicine
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Nagamune Teruyuki
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo:dep
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Nakagawa Hideyuki
Department Of Anesthesiology Saitama International Medical Center Saitama Medical University
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Kitayama A
Department Of Chemistry And Biotechnology Graduate School Of Engineering University Of Tokyo:(presen
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Kitayama Atsushi
Department Of Chemistry And Biotechnology The University Of Tokyo
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Arai Ryoichi
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Ueda Hiroshi
Department Of Anatomy University Of Occupational And Environmental Health School Of Medicine
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Nakagawa Hideyuki
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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Ueda Hiroshi
Department Of Anatomy School Of Medicine University Of Occupational And Environmental Health
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