Open Sandwich Immunoassay : a Novel Immunoassay Approach Based on the Interchain Interaction of an Antibody Variable Region(MEDICAL BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
Despite their general applicability, conventional sandwich immunoassays suffer from the requirements of two antibodies recognizing different epitopes, and multiple long incubation/washing steps. In addition, the assay is not applicable to the measurement of low-molecular-weight haptens due to their size. Recently, a novel sandwich-type immunoassay that can overcome the limitations of conventional sandwich immunoassays was proposed. The assay, which utilizes antigen-dependent stabilization of an antibody variable region, requires less time and handling, and is particularly suitable for hapten measurement. The principle could be applied to several homogeneous assay formats, and the method has been shown to have superior sensitivity, as well as a lower time requirement compared to conventional assays.
- 公益社団法人日本生物工学会の論文
- 2002-12-25
著者
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UEDA Hiroshi
Department of Pathology, School of Medicine Gifu University
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Ueda H
Field Sci. Ctr. Hokkaido Univ.
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Ueda Hiroshi
Department Of Anatomy University Of Occupational And Environmental Health School Of Medicine
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Ueda Hiroshi
Dep. Of Chemistry And Biotechnology School Of Engineering Univ. Of Tokyo
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Ueda Hiroshi
Department Of Anatomy School Of Medicine University Of Occupational And Environmental Health
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