Method for Obtaining Mutants Defective in Immunoglobulin μ Transcription Factors
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概要
- 論文の詳細を見る
A method of obtaining mutants defective in the regulatory function of Ig μ gene expression was developed. Such mutants are useful for discovering the functions of transcription factors and isolating their genes, especially those of DNA-unbinding factors. Cells expressing Ig μ and Eco-GPT simultaneously under control of each μ enhancer were prepared as follows: myeloma X63Ag8.653 cells were transfected with pSV-VμMeΔCH1 encoding a μ gene modified for cell surface μ expression without light chains, selected by neomycin resistance, transfected with the Eco-GPT gene connected to the μ enhancer, and selected with mycophenolic acid in a medium containing xanthine. The μ_m/Eco-GPT cells were mutated with ethane methyl sulfonate (EMS), and selected with toxin-conjugated anti-μ antibody, and then with 6-thioguanine. The μ_m^-/Eco-GPT^- mutants obtained were fused with X63Ag8.653 cells. Fusion caused the mutants to recover μ_m expression, suggesting that some trans-acting transcription factor other than the μ-encoding gene itself was probably mutated.
- 社団法人日本農芸化学会の論文
- 1996-11-23
著者
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Suzuki E
Shinshu Univ. Ueda Jpn
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SUZUKI Eiji
TDK Corporation
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Suzuki Eiji
Department Of Fine Materials Engineering Faculty Of Textile Science & Technology Shinshu Univers
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Ueda Hiroshi
Department Of Dermatology Fujita Health University School Of Medicine
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Nishimura H
Department Of Bioscience And Technology School Of Engineering Hokkaido Tokai University
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Suzuki E
Tdk Corporation
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Shindo Takuya
Engineering Research Laboratories Research Institute
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Nishimura Hajime
Department Of Chemical Engineering Faculty Of Engineering University Of Tokyo
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MAKISHIMA Fusao
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
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Suzuki Eiji
Department Of Breast Surgery Tokyo Metropolitan Komagome Hospital
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Makishima Fusao
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo:pha
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Suzuki Eiji
Department Of Biochemistry Faculy Of Veterinary Medicine Hokkaido University
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Ueda Hiroshi
Department Of Anatomy University Of Occupational And Environmental Health School Of Medicine
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Ueda Hiroshi
Department Of Anatomy School Of Medicine University Of Occupational And Environmental Health
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