Antigen-Mediated Genetically Modified Cell Amplification (AMEGA) with Single Vector Transduction

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概要

• 論文の詳細を見る

• Previously, we proposed the Antigen-mediated genetically modified cell amplification (AMEGA) system, which can selectively amplify gene-transduced cells without antibiotic selection. While the original AMEGA system employed two vectors encoding a pair of antibody/receptor chimeras and a gene of interest, the use of two virions resulted in low co-transduction efficiency and time-consuming selection. Here we show an improved AMEGA system, where the three genes are linked in tandem with internal ribosomal entry sites in a single vector. We used gp130 chimeras whose extracellular domain was replaced with either VH or VL region of anti-hen egg lysozyme (HEL) antibody HyHEL-10 and D2 domain of erythropoietin receptor. The constructed vector was retrovirally transduced to IL-3-dependent Ba/F3 cells followed by HEL selection in the absence of IL-3. The resultant transduction efficiency increased by ~100-fold compared to the previous two-vector system, which results in a much shorter selection period.

• 社団法人 化学工学会の論文
• 2004-10-01

著者

• UEDA Hiroshi

  Department of Pathology, School of Medicine Gifu University

• Nagamune Teruyuki

  Department of Bioengineering, Graduate School of Engineering, The University of Tokyo

• KAWAHARA MASAHIRO

  Department of Medicine and Clinical Science, Graduate School of Medicine, Kyoto University

• Tsumoto Kouhei

  東京大学 工学系研究科化学生命工学専攻

• Tsumoto K

  Department Of Medical Genome Sciences Graduate School Of Frontier Sciences The University Of Tokyo

• Tsumoto Kouhei

  Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University

• Nagamune T

  Univ. Tokyo Tokyo Jpn

• Nagamune T

  Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo

• Nagamune Teruyuki

  Dep. Of Chemistry And Biotechnology Graduate School Of Engineering The Univ. Of Tokyo 7-3-1 Hongo Bu

• Nagamune Teruyuki

  Department Of Bioengineering Graduate School Of Engineering The University Of Tokyo:department Of Ch

• Nagamune Teruyuki

  Department Of Bioengineering Center For Nanobio Integration The University Of Tokyo:department Of Ch

• Kumagai Izumi

  Department of Biomolecular Engineering, Tohoku University

• 上田 宏

  東京大学大学院工学研究科

• Kawahara Masahiro

  Department Of Chemistry And Biotechnology School Of Engineering

• Kawahara Masahiro

  Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences The University Of Tokyo

• Kumagai Izumi

  Department Of Biomolecular Engineering Tohoku University

• Kumagai Izumi

  Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University

• Kumagai Izumi

  Department Of Biochemistry And Engineering Graduate School Of Engineering Tohoku University

• Kumagai I

  Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University

• Ueda Hiroshi

  Department Of Dermatology Fujita Health University School Of Medicine

• KANEKO Etsuji

  Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo

• Nagamune Teruyuki

  Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo:dep

• Kaneko Etsuji

  Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo

• Kumagai Izumi

  Dept Biomol Eng Grad Sch Eng Tohoku Univ.

• Ueda Hiroshi

  Department Of Anatomy University Of Occupational And Environmental Health School Of Medicine

• Ueda Hiroshi

  Department Of Anatomy School Of Medicine University Of Occupational And Environmental Health

• Kawahara Masahiro

  Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo

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