Effects of Mutation in the Conserved GTSRH Sequence of the Motor Protein Prestin on Its Characteristics(<Special Issue>Bioengineering)
スポンサーリンク
概要
- 論文の詳細を見る
Prestin is a motor protein responsible for the outer hair cell (OHC) electromotility which amplifies the vibration of the organ of Corti in the inner ear. Identification of the functional significance of particular amino acids is necessary to characterize prestin. In this study, an attempt was made to clarify the role of the GTSRH sequence at positions 127-131 in prestin conserved in six proteins of the solute carrier (SLC) 26 family of which prestin is a member. To elucidate what role that sequence plays in the characteristics of prestin, mutations were introduced into the sequence and the characteristics of the constructed point mutants were investigated by Western blotting, immunofluorescence experiments and the whole-cell patchclamp technique. The localization of T128A was altered, the anion transport function of H131A and that of S129T were lost and such functions of G127A, T128A, S129A and R130A declined. These results suggest that the GTSRH sequence plays an important role in the localization of prestin, as well as in its anion transport function.
- 社団法人日本機械学会の論文
- 2005-12-15
著者
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KOBAYASHI Toshimitsu
Department of Otolaryngology, Nagasaki University School of Medicine
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Kobayashi Toshimitsu
Department Of Cardiovascular Surgery Tohoku University Graduate School Of Medicine
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IKEDA Katsuhisa
Department of Otorhinolaryngology, Head and Neck Surgery, Tohoku University School of Medicine
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WADA HIROSHI
Department of Surgery, Graduate School of Medicine, Osaka University
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Wada Hiroshi
Department Of Cardiovascular Science And Medicine Chiba University Graduate School Of Medicine
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Tsumoto Kouhei
東京大学 工学系研究科化学生命工学専攻
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Tsumoto K
Department Of Medical Genome Sciences Graduate School Of Frontier Sciences The University Of Tokyo
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Tsumoto Kouhei
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Iida Koji
Department of Bioengineering and Robotics, Tohoku University
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Murakoshi Michio
Department of Bioengineering and Robotics, Tohoku University
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Kumano Shun
Department of Bioengineering and Robotics, Tohoku University
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Kumagai Izumi
Department of Biomolecular Engineering, Tohoku University
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NAITO Naoyuki
Department of Bioengineering and Robotics, Tohoku University
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Tabayashi Koichi
Department Of Cardiovascular Surgery Tohoku University Graduate School Of Medicine
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Kumagai Izumi
Department Of Biomolecular Engineering Tohoku University
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Kumagai Izumi
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Kumagai Izumi
Department Of Biochemistry And Engineering Graduate School Of Engineering Tohoku University
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Kobayashi T
Department Of Cardiovascular Surgery Tohoku University Graduate School Of Medicine
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Kumagai I
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Iida Koji
Department Of Bioengineering And Robotics Tohoku University
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Iida Koji
Department Of Applied Physics National Defence Academy
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Kumano Shun
Department Of Bioengineering And Robotics Tohoku University
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Koike Takuji
Department Of Mechanical Engineering And Intelligent Systems The University Of Electro-communication
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Wada H
Department Of Bioengineering And Robotics Tohoku University
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Naito Naoyuki
Department Of Bioengineering And Robotics Tohoku University
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Ikeda Katsuhisa
Department Of Otolaryngology
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Murakoshi Michio
Department Of Bioengineering And Robotics Tohoku University
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Ikeda Katsuhisa
Department Of Otorhinolaryngology Head And Neck Surgery Tohoku University School Of Medicine
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Kumagai Izumi
Dept Biomol Eng Grad Sch Eng Tohoku Univ.
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Wada Hiroshi
Department Of Bioengineering And Robotics Tohoku University
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Koike Takuji
Department Of Mechanical Engineering And Intelligent Systems The University Of Electro-communication
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Ikeda Katsuhisa
Department of Bioengineering and Robotics, Tohoku University
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