Stable Expression of the Motor Protein Prestin in Chinese Hamster Ovary Cells(<Special Issue>Bioengineering)
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概要
- 論文の詳細を見る
Mammalian hearing sensitivity relies on a mechanical amplification mechanism involving the outer hair cells (OHCs), which rapidly alter their longitudinal length in response to changes in their membrane potential. The molecular basis of this mechanism is thought to be a motor protein embedded in the lateral membrane of the OHCs. Recently, this motor protein was identified and termed prestin. Since then, prestin has been researched intensively to elucidate the behavior of the OHCs. However, little progress in the study of prestin at the molecular level has been made because no method of obtaining an adequate amount of prestin has been established. In this study, therefore, an attempt was made to construct a stable expression system of prestin using Chinese hamster ovary (CHO) cells. The expression of prestin in the transfected CHO cells and the activity of prestin on CHO cells were confirmed by immunofluorescence and whole-cell patch-clamp measurements, respectively.
- 社団法人日本機械学会の論文
- 2003-12-15
著者
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KOBAYASHI Toshimitsu
Department of Otolaryngology, Nagasaki University School of Medicine
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Kobayashi Toshimitsu
Department Of Cardiovascular Surgery Tohoku University Graduate School Of Medicine
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OSHIMA Takeshi
Department of Otorhinolaryngology, Head and Neck Surgery, Tohoku University School of Medicine
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KOJIMA Kanako
Department of Medical Genetics, Tohoku University School of Medicine
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IKEDA Katsuhisa
Department of Otorhinolaryngology, Head and Neck Surgery, Tohoku University School of Medicine
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WADA HIROSHI
Department of Surgery, Graduate School of Medicine, Osaka University
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Oshima Takeshi
Department Of Otorhinolaryngology Head And Neck Surgery Tohoku University School Of Medicine
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Oshima Takeshi
東北大学 医学部遺伝病学
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Oshima Takeshi
Department Of Otorhinolaryngology Tohoku University School Of Medicine
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Wada Hiroshi
Department Of Cardiovascular Science And Medicine Chiba University Graduate School Of Medicine
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Kojima Kanako
Department Of Medical Genetics Tohoku University School Of Medicine
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Tsumoto Kouhei
東京大学 工学系研究科化学生命工学専攻
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Tsumoto K
Department Of Medical Genome Sciences Graduate School Of Frontier Sciences The University Of Tokyo
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Tsumoto Kouhei
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Iida Koji
Department of Bioengineering and Robotics, Tohoku University
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Kumagai Izumi
Department of Biomolecular Engineering, Tohoku University
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KONNO Kazuaki
Department of Bioengineering and Robotics, Tohoku University
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Tabayashi Koichi
Department Of Cardiovascular Surgery Tohoku University Graduate School Of Medicine
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Kumagai Izumi
Department Of Biomolecular Engineering Tohoku University
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Kumagai Izumi
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Kumagai Izumi
Department Of Biochemistry And Engineering Graduate School Of Engineering Tohoku University
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Oshima Takeshi
Department Of Otolaryngology Tohoku University School Of Medicine
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Kobayashi T
Department Of Cardiovascular Surgery Tohoku University Graduate School Of Medicine
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Kumagai I
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Iida Koji
Department Of Bioengineering And Robotics Tohoku University
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Iida Koji
Department Of Applied Physics National Defence Academy
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Koike Takuji
Department Of Mechanical Engineering And Intelligent Systems The University Of Electro-communication
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Wada H
Department Of Bioengineering And Robotics Tohoku University
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Konno Kazuaki
Department Of Bioengineering And Robotics Tohoku University
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Ikeda Katsuhisa
Department Of Otolaryngology
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Ikeda Katsuhisa
Department Of Otorhinolaryngology Head And Neck Surgery Tohoku University School Of Medicine
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Kumagai Izumi
Dept Biomol Eng Grad Sch Eng Tohoku Univ.
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Wada Hiroshi
Department Of Bioengineering And Robotics Tohoku University
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Koike Takuji
Department Of Mechanical Engineering And Intelligent Systems The University Of Electro-communication
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Ikeda Katsuhisa
Department of Bioengineering and Robotics, Tohoku University
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