Bone regeneration using collagen type I vitrigel with bone morphogenetic protein-2(MEDICAL BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
Bone morphogenetic protein-2 is a very promising candidate for the treatment of bone diseases and defects, but more effective therapeutic methods are required due to its instability in vivo. A controlled and localized delivery system of Bone morphogenetic protein-2 would be appropriate for effective bone regeneration. Here, we report a novel delivery system of bone morphogenetic protein-2 using vitrigel(a novel stable collagen gel membrane prepared from vitrified type I collagen) for in vivo bone regeneration. Scanning electron microscopy revealed that the collagen vitrigel formed a tightly woven network with average pore sizes of about 1-2μm. The vitrigel scaffold delivery system exhibited sustained release of bone morphogenetic protein-2 and >80% of the total bone morphogenetic protein-2 was still retained in the vitrigel after 15 days in phosphate-buffered saline in vitro. Bone morphogenetic protein-2-containing vitrigel was transplanted into mouse calvarial defects. The enhanced mechanical strength of the vitrigel made it easier to implant into defects without damage. Obvious bone regeneration was observed in the defects of mice treated with as little as 0.19μg of bone morphogenetic protein-2 at 4 weeks after the transplantation. The local and sustained delivery system for bone morphogenetic protein-2 developed in the present study may represent a powerful modality for bone regeneration.
- 社団法人日本生物工学会の論文
著者
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Nagamune Teruyuki
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo
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Chung Ung‐il
Univ. Tokyo Hospital Tokyo Jpn
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SHINKAI Masashige
Department of Biotechnology, Graduate School of Engineering, Nagoya university
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Chung Ung-il
Division Of Tissue Engineering University Of Tokyo Hospital
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Chung Ung-il
Division Of Tissue Engineering Faculty Of Medicine The University Of Tokyo
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Chung Ung-il
Center For Disease Biology And Integrated Medicine School Of Medicine University Of Tokyo:department
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Zhao Jiyuan
Department of Chemistry and Biotechnology, School of Engineering, University of Tokyo
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Takezawa Toshiaki
Laboratory of Animal Cell Biology, National Institute of Agrobiological Sciences
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Ohba Shinsuke
Center for Disease Biology and Integrated Medicine, School of Medicine, University of Tokyo
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Nagamune Teruyuki
Department Of Bioengineering Center For Nanobio Integration The University Of Tokyo:department Of Ch
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Shinkai Masashige
Department Of Chemistry And Biotechnology School Of Engineering University Of Tokyo
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Ohba Shinsuke
Center For Disease Biology And Integrated Medicine School Of Medicine University Of Tokyo
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Takezawa Toshiaki
Laboratory Of Animal Cell Biology National Institute Of Agrobiological Sciences
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Zhao Jiyuan
Department Of Chemistry And Biotechnology School Of Engineering University Of Tokyo
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Nagamune Teruyuki
Department Of Chemistry And Biotechnology School Of Engineering University Of Tokyo:department Of Bi
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Shinkai Masashige
Department of Biotechnology, Faculty of Engineering, Nagoya University
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