Changes in Bone Regeneration by Trehalose Coating and Basic Fibroblast Growth Factor after Implantation of Tailor-Made Bone Implants in Dogs
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概要
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In this study, we aimed to determine the effect of trehalose coating and the optimal dose of basic fibroblast growth factor (bFGF), an osteoinductive protein, loaded onto tailor-made bone implants for implant-induced bone formation in vivo. We fabricated tailor-made α-tricalcium phosphate bone implants (11 mm diameter with 2 parallel cylindrical holes). bFGF 0, 1, 10, 100 or 200 μg/implant was incorporated into implants with and without a trehalose coating, and these were subsequently implanted into dogs to correct temporal bone defects of the same size and shape. Four weeks after implantation, we analyzed the bone implants and surrounding tissues by using micro-computed tomography imaging and histological analyses, as well as gross evaluation. No significant difference in new bone formation was observed between implants with and without trehalose coating at any of the bFGF doses. Bone implants with 100 and 200 μg bFGF showed significantly more new bone formation at the implant site and within the cylindrical holes of the implants than those without bFGF (P<0.05). However, heterotopic bone formation on the skull near the implant was observed in the group that received 200 μg bFGF. These results suggest that 100 μg bFGF is the optimal dose for this implant in dogs, and that the trehalose coating may not be necessary in vivo, probably due to the presence of blood proteins and electrolytes at the implant site.
著者
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Chung Ung-il
Division Of Tissue Engineering Faculty Of Medicine The University Of Tokyo
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IGAWA Kazuyo
Division of Tissue Engineering, University of Tokyo Hospital
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MOCHIZUKI Manabu
Laboratory of Veterinary Diagnostic Imaging, Department of Veterinary Medicine, Tokyo University of
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SUZUKI Shigeki
NEXT 21, K.K.
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SASAKI Nobuo
Laboratory of Surgery, Veterinary Medical Science, The University of Tokyo
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CHOI Sungjin
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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LEE Jongil
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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LIU I-Li
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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HONNAMI Muneki
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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LIU I-Li
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, the University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
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LEE Jongil
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, the University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
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MOCHIZUKI Manabu
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, the University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
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NISHIMURA Ryohei
NEXT 21 K.K., 3–38–1 Hongo, Bunkyo-ku, Tokyo 113–0033, Japan
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IGAWA Kazuyo
Division of Tissue Engineering, University of Tokyo Hospital, 7–3–1 Hongo, Bunkyo-ku, Tokyo 113–0033, Japan
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SASAKI Nobuo
Laboratories of Veterinary Surgery, Division of Veterinary Medical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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HONNAMI Muneki
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, the University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
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LLE Jongil
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, the University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
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LIU I-li
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
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HONNAMI Muneki
Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
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