Retinoids Induce Growth Inhibition and Apoptosis in Mast Cell Tumor Cell Lines

スポンサーリンク

概要

• 論文の詳細を見る

• Retinoids are well recognized as promising antitumor agents in humans. However, there have only been a few reports about the effect of retinoids in canine cancers. To investigate the antitumor effect of retinoids on mast cell tumors (MCT), inhibitory effect on cell growth and induction of apoptosis were examined in vitro. Although sensitivity of these cells differed among the cells, the growth of three MCT cell lines (CoMS, CM-MC and VI-MC) were inhibited dose dependently when they were treated with retinoids. FACS analysis of PI-stained nuclei revealed an apoptotic fraction in CM-MC cells about 30% when treated with retinoids, while those of control cells were less than 5%. Caspase-3 activation was observed after retinoid treatment in CM-MC cells. This was confirmed by inhibiting the retinoid-induced apoptosis using the pan-caspase inhibitor, ZVAD-FMK. Both retinoid receptors, RARs and RXRs, were detected by immunoprecipitation followed by western blot analysis in all the three MCT cells. These data suggests that retinoids inhibit the growth of MCTs partly through apoptosis, and this growth inhibition by retinoids may be mediated by RARs and RXRs. We conclude that retinoid may be a potential adjunctive chemotherapeutic agent for the treatment of canine MCT.

著者

• MOCHIZUKI Manabu

  Laboratory of Veterinary Diagnostic Imaging, Department of Veterinary Medicine, Tokyo University of

• Miyajima Nozomi

  Laboratory Of Veterinary Surgery Graduate School Of Agricultural And Life Sciences University Of Tok

• Nakagawa Takayuki

  Laboratory Of Developmental Immunology And Crest Program Of Japan Science And Technology Agency Grad

• Kagechika Hiroyuki

  School Of Biomedical Science Institute Of Biomaterials And Bioengineering Tokyo Medical And Dental U

• Takahashi Tomoko

  Department Of Cancer Cytogenetics Division Of Molecular Biology Research Institute For Radiation Bio

• SASAKI Nobuo

  Laboratory of Surgery, Veterinary Medical Science, The University of Tokyo

• NISHIMURA Ryohei

  Laboratory of Emergency Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo

• OHASHI Emi

  Laboratory of Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo

関連論文

• Trehalose May Prevent Postsurgical Adhesions in a Rabbit Model of Hysterotomy
• Enhancement of lung carcinogenesis initiated with 4-(N-hydroxymethylnitrosamino)-1-(3-pyridyl)-1-butanone by Ogg1 gene deficiency in female, but not male, mice
• Maxillofacial reconstruction using custom-made artificial bones fabricated by inkjet printing technology
• Tailor-made tricalcium phosphate bone implant directly fabricated by a three-dimensional ink-jet printer
• Chromosomal Instability in Acute Myelocytic Leukemia and Myelodysplastic Syndrome Patients among Atomic Bomb Survivors
• Cytotoxic C-Benzylated Dihydrochalcones from Uvaria acuminata
• Detection of Human Papillomavirus Type 16 in Tear Fluid Before Conjunctival Neoplasia Excision
• Peroxisome proliferator-activated receptor γ ligands stimulate myeloid differentiation and lipogenensis in human leukemia NB4 cells
• Biocompatibility of two different types of extracellular matrix in bioartificial endocrine pancreas in dogs: a preliminary report
• Bone regeneration within a tailor-made tricalcium phosphate bone implant with both horizontal and vertical cylindrical holes transplanted into the skull of dogs
• Manganese Superoxide Dismutase Is Induced by Endoplasmic Reticulum Stress through IRE1-Mediated Nuclear Factor (NF)-κB and AP-1 Activation(Biochemistry/Molecular Biology)
• Tumor Cell Growth Inhibition and Cell Differentiation Analysis in a Canine Mammary Tumor Cell Line (MCM-B2) Treated with Four Chemical Reagents
• A Case of Abnormal Growth Hormone Secretion Possibly Caused by Low Somatomedin Production of Unknown Origin:Is The Unresponsiveness of Gh Receptor Responsible For It?
• An Interspecies Comparison of Placental Gangliosides
• Pseudotumor Cerebri Induced by Minocycline Therapy for Acne Vulgaris
• Neoplasitic Diseases in Ferrets in Japan: A Questionnaire Study for 2000 to 2005
• Microwave Zeeman Effect of Ketene
• Hypoxia-Inducible Factor-3α Functions as an Accelerator of 3T3-L1 Adipose Differentiation
• IL-6 positively regulates Foxp3^+CD8^+ T cells in vivo
• Effect of Trehalose Coating on Basic Fibroblast Growth Factor Release from Tailor-Made Bone Implants
• Effects of NF-κB Expression and Its Inhibition on Canine Mammary Cancer Cell Lines Using an Immunodeficient Mice Model
• Comparison of Behavioral Effects of Morphine and Fentanyl in Dogs and Cats
• Docosahexaenoic Acid Induces Adipose Differentiation-Related Protein through Activation of Retinoid X Receptor in Human Choriocarcinoma BeWo Cells
• Retinoids Induce Growth Inhibition and Apoptosis in Mast Cell Tumor Cell Lines
• Comparison of Feasibility and Safety of Epidural Catheterization between Cranial and Caudal Lumbar Vertebral Segments in Dogs
• A Derivation of Generalized Semi-Classical Langevin Equations Based on the Feynman Path-Integral Approach
• c-Jun N-terminal kinase activation by oxidative stress suppresses retinoid signaling through proteasomal degradation of retinoic acid receptor α protein in hepatic cells
• Significance of Tumor Infiltrating Immune Cells in Spontaneous Canine Mammary Gland Tumor: 140 cases
• A case of a 6-year-old girl with anti-neutrophil cytoplasmic autoantibody-negative pauci-immune crescentic glomerulonephritis
• Adhesional Function of Canine Mammary Gland Tumor Cells Expressing Sialyl Lewis X
• Physical Properties of Semi-Liquid Food and Swallowing Behaviors through Measurement of Electromyography from Suprahyoid Musculatures during Swallowing
• Acute Improvement by Pravastatin of Endothelial Function in Normolipidemic Males with Cardiovascular Risk Factors
• Relationship between NF-κB Expression and Malignancy of Canine Mammary Gland Tumor Tissues
• Genome-wide association study to identify genetic variants present in Japanese patients harboring intracranial aneurysms
• Repeated Treatment with Furazolidone Induces Multiple Cytochrome P450-Related Activities in Chicken Liver, but not in Rat Liver
• Changes in Bone Regeneration by Trehalose Coating and Basic Fibroblast Growth Factor after Implantation of Tailor-Made Bone Implants in Dogs
• A Comparison of Neurosphere Differentiation Potential of Canine Bone Marrow-Derived Mesenchymal Stem Cells and Adipose-Derived Mesenchymal Stem Cells
• Expression of the MDR1 Gene and P-Glycoprotein in Canine Mast Cell Tumor Cell Lines
• Distribution of Contrast Medium Epidurally Injected at Thoracic and Lumbar Vertebral Segments
• Implantation of Tetrapod-Shaped Granular Artificial Bones or β-Tricalcium Phosphate Granules in a Canine Large Bone-Defect Model
• Cardiovascular Reflex Mechanisms by Topical Instillation of Capsaicin and Distilled Water into the Larynx in Anesthetized Dogs.
• Repair of Ulnar Segmental Defect by Recombinant Human Bone Morphogenetic Protein-2 in Dogs.
• Establishment and Characterization of Two Cell Lines Derived from Canine Spontaneous Osteosarcoma.
• Xenotransplantation of Porcine Pancreatic Endocrine Cells to Total Pancreatectomized Dogs
• Aryliminodimagnesium reagents. III. The deoxygenative condensation with nitroso- and nitroarenes: Electronic effect of substituents on product distribution.
• Interdigitating Cell Sarcoma-A report of an autopsy case and literature review-:—A report of an autopsy case and literature review—
• The steric hindrance of the stepwise reaction of N-carboxy .ALPHA.-amino acid anhydride with the .ALPHA.-amino acid ester.

もっと見る 閉じる

スポンサーリンク