Expression of the MDR1 Gene and P-Glycoprotein in Canine Mast Cell Tumor Cell Lines
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概要
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Cellular drug resistance to antineoplastic drugs is often due to the presence of a drug efflux pump that reduces intracellular drug accumulation and chemosensitivity. P-glycoprotein (P-gp), which is encoded by the MDR1 gene, is considered to function as an ATP-driven membrane drug efflux pump and appears to play an important role in tumor cell resistance. In the present report, we assessed the expression of MDR1 by RT-PCR in three canine mast cell tumor cell lines, TiMC, CoMS and LuMC, originating from a cutaneous tumor, an oral-mucosal tumor and a gastrointestinal tumor, respectively. P-gp expression was also examined by Western blot analysis, while the functional activity of P-gp was assessed by flowcytometric analysis of intracellular rhodamine-123 (Rhd-123) uptake. The results revealed that MDR1 gene and P-gp were both expressed in CoMS and LuMC cells, whereas neither was present in TiMC cells. In CoMS and LuMC cells, intracellular uptake of Rhd-123 increased in the presence of verapamil, a functional modulator of P-gp. In contrast, TiMC cells did not show any changes in the intracellular accumulation of Rhd-123 after the verapamil addition. These findings suggest that the expressions of MDR1 gene and P-gp probably contribute to cellular drug resistance in canine mast cell tumors.
- 公益社団法人 日本獣医学会の論文
著者
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Okuda Masaru
Department Of Cell Biology Neurobiology And Anatomy University Of Cincinnati College Of Medicine
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TAKAHASHI Tomoko
Laboratory of Veterinary Radiology, Department of Veterinary Medicine, College of Bioresource Scienc
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Kadosawa Tsuyoshi
Department Of Small Animal Clinical Sciences School Of Veterinary Medicine Rakuno Gakuen University
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Taura Yasuho
Department Of Veterinary Hospital Faculty Of Agriculture Yamaguchi University
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SASAKI Nobuo
Laboratory of Surgery, Veterinary Medical Science, The University of Tokyo
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NAKAMOTO Yuya
Department of Veterinary Surgery, Yamaguchi University
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ITAMOTO Kazuhito
Department of Veterinary Surgery, Yamaguchi University
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ITAMOTO Kazuhito
Department of Animal Medical Center, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan
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NAKAICHI Munekazu
Department of Veterinary Surgery, Yamaguchi University
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TAKESHITA Yoko
Department of Veterinary Surgery, Yamaguchi University
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UNE Satoshi
Department of Veterinary Hospital, Yamaguchi University
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OKUDA Masaru
Department of Veterinary Internal Medicine, Yamaguchi University
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KADOSAWA Tsuyoshi
Department of Companion Animal Clinical Science, School of Veterinary Medicine, Rakuno Gakuen University
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UNE Satoshi
Department of Veterinary Hospital, Faculty of Agriculture, Yamaguchi University
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SASAKI Nobuo
Laboratories of Veterinary Surgery, Division of Veterinary Medical Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo
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