Xenotransplantation of Porcine Pancreatic Endocrine Cells to Total Pancreatectomized Dogs
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概要
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Xenotransplantation of porcine pancreatic endocrine (PE) cells in a diffusion chamber, a bioartificial endocrine pancreas (Bio-AEP), was conducted to total pancreatectomized dogs. Six pancreatectomized dogs were divided into two groups of 3 dogs each. In three dogs of the control group, exogenous insulin was administered twice a day for 30 weeks to maintain fasting blood glucose (FBG) levels within the normal range. The remaining three dogs were implanted with Bio-AEPs (implantation group), in addition to daily insulin administration. In the implantation group, Bio-AEPs containing 1.3 to 1.8 × 107 cells per kg of body weight of the recipient were implanted without fixation into the abdominal cavity. In the control group, exogenous insulin requirements did not decrease during the experimental period, whereas it significantly decreased for a certain period (3, 11, 17 weeks) after implantation in all implanted dogs. In the implantation group, laparotomy was performed after FBG and the exogenous insulin requirement increased again and Bio-AEPs were removed. Two Bio-AEPs were completely destroyed, and the remaining one was encapsulated by thin fibrous tissue. In this dog, effusion was present within the capsule, but the Bio-AEP was not destroyed. Histopathologically, the necrosis, presumably caused by hypoxia, of the PE-cells was observed on transmission electron microscopy. In conclusion, Bio-AEP could function for a certain period after implantation in this study. However, more preclinical researches should be needed to apply this technique for the treatment of diabetic dogs.
- 公益社団法人 日本獣医学会の論文
著者
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NASU Koko
Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medici
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IWAMI Yukiko
Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medici
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EDAMURA Kazuya
Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medici
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OHGAWARA Hisako
Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medici
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Ogawa Hiroyuki
Laboratory Of Veterinary Emergency Medicine
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Itakura Shin
Laboratory Of Veterinary Surgery Graduate School Of Agricultural And Life Sciences University Of Tok
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NASU Koko
Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medicine, Tokyo Women's Medical University
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IWAMI Yukiko
Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medicine, Tokyo Women's Medical University
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SASAKI Nobuo
Laboratory of Surgery, Veterinary Medical Science, The University of Tokyo
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