Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
- 論文の詳細を見る
Streptococcus pyogenes sortase A (SpSrtA) was transduced into mammalian cells. Then SpSrtA-mediated intracellular circularization of a model protein was confirmed in a time and dose-dependent manner by Western blotting analysis. Direct transduction of SpSrtA is expected to be a strong tool for more conditional specific protein modification in mammalian cells.
著者
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Nagamune Teruyuki
Department Of Bioengineering Center For Nanobio Integration The University Of Tokyo:department Of Ch
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Hirakawa Hidehiko
Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo
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Yamaguchi Satoshi
Department Of Agricultural Chemistry Faculty Of Agriculture Kyushu University
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Zhang Jing
Department Of Anatomy Histology & Embryology School Of Basic Medical Sciences Peking University
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Yamaguchi Satoshi
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
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Hirakawa Hidehiko
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
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Zhang Jing
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo
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