Cloning and Characterization of Extradiol Aromatic Ring-Cleavage Dioxygenases of Pseudomonas aeruginosa JI104

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概要

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• We have cloned multiple extradiol aromatic ring-cleavage dioxygenase (EDO) genes from a gene library of Pseudomonas aeruginosa JI104,which is abenzene degrader isolated from soil near a gasworks. Southern hybridization analysis revealed that P. aeruginosa JI104 possessed three homologous catechol 2,3-dioxygenase (C23O) genes. Nucleotide sequences of the cloned C23O genes, xylE_<JI104-1,2,3> were almost identical to that of the archetypal C23O gene (xylE_<TOL>l;), which is carried on the TOL plasmid, pWW0. We also cloned another EDO gene, bphC_<JI104> the product of which showed less activity for catechol than did XylE_<JI104> but higher activity for 2,3-dihydroxy biphenyl. The nucleotide sequence of bphC_<JI104> was identical to that of bphC_<KF707> (2,3-dihydroxybiphenyl dioxygenase gene of Pseudomonas pseudoalcaligenes KF707). The substrate specificities of the four EDOs of P. aeruginosa JI104 were markedly different from each other. Although XylE_<JI104-1> and XylE_<TOL> were 94% homologous, the specificities of the gene products for 4-chlorocatechol were extremely different. Results of a study of the chimeric enzymes composed of XylE_<JI104-1> and XylE_<TOL> N- and C-terminal regions showed that the difference in the specificity for 4-chlorocatechol was dependent on the C-terminal amino acid sequences. All of the isofunctional homologous EDOs in P. aeruginosa JI104 seem to have been derived from a common ancestor and evolved into the present forms in which each EDO is involved in a different degradation pathway and they all coexist in one strain.

• 社団法人日本生物工学会の論文
• 1996-09-25

著者

• UEDA Hiroshi

  Department of Pathology, School of Medicine Gifu University

• Nagamune Teruyuki

  Department of Bioengineering, Graduate School of Engineering, The University of Tokyo

• Suzuki Eiji

  Department of Internal Medicine II, Fukushima Medical University School of Medicine, Fukushima

• Suzuki E

  Shinshu Univ. Ueda Jpn

• Kawakami Yasushi

  Department Of Chemical Engineering University Of Tokyo

• Nagamune T

  Univ. Tokyo Tokyo Jpn

• Nagamune T

  Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo

• Nagamune Teruyuki

  Dep. Of Chemistry And Biotechnology Graduate School Of Engineering The Univ. Of Tokyo 7-3-1 Hongo Bu

• Nagamune Teruyuki

  Department Of Bioengineering Center For Nanobio Integration The University Of Tokyo:department Of Ch

• SUZUKI Eiji

  TDK Corporation

• KITAYAMA ATSUSHI

  Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo

• Suzuki Eiji

  Department Of Fine Materials Engineering Faculty Of Textile Science & Technology Shinshu Univers

• Kikuchi Masako

  (present Address)institute Of Research And Innovation Biotechnology Department

• Kawakami Yasushi

  (present Address)institute Of Research And Innovation Biotechnology Department

• ACHIOKU TOMOHISA

  Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo

• YANAGAWA TAKAHIRO

  Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo

• KANOU KENJIRO

  Department of Chemistry and Biotechnology, Graudate School of Engineering, The University of Tokyo

• NISHIMURA HAJIME

  (Present address)The Research Studio "Synthesis"

• Kanou Kenjiro

  Department Of Chemistry And Biotechnology Graudate School Of Engineering The University Of Tokyo

• Ueda Hiroshi

  Department Of Dermatology Fujita Health University School Of Medicine

• Nishimura H

  Department Of Bioscience And Technology School Of Engineering Hokkaido Tokai University

• Suzuki E

  Tdk Corporation

• Achioku Tomohisa

  Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo

• Yanagawa Takahiro

  Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo

• Kitayama A

  Department Of Chemistry And Biotechnology Graduate School Of Engineering University Of Tokyo:(presen

• Kitayama Atsushi

  Department Of Chemistry And Biotechnology The University Of Tokyo

• Suzuki Eiji

  Department Of Fine Mateials Engineering Faculty Of Textile Science And Technology Shinshu University

• Suzuki Eiji

  Department Of Breast Surgery Tokyo Metropolitan Komagome Hospital

• Suzuki Eiji

  Department Of Biochemistry Faculy Of Veterinary Medicine Hokkaido University

• Ueda Hiroshi

  Department Of Anatomy University Of Occupational And Environmental Health School Of Medicine

• Ueda Hiroshi

  Department Of Anatomy School Of Medicine University Of Occupational And Environmental Health

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